Deoxyadenosine metabolism and toxicity in cultured L5178Y cells

The growth of cultured L5178Y cells is inhibited by relatively low concentrations fo deoxyadenosine in the presence of deoxycoformycin, an inhibitor of adenosine deaminase. Cell viability is reduced, presumably as a consequence of the induced state of unbalanced growth which is characterized by inhi...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1977-09, Vol.37 (9), p.3013
Main Authors: Lowe, J K, Gowans, B, Brox, L
Format: Article
Language:English
Subjects:
Adenosine Deaminase Inhibitors
Animals
Azepines - pharmacology
Cell Division - drug effects
Cell Survival - drug effects
Cells, Cultured
Deoxyadenosines - metabolism
Deoxyadenosines - pharmacology
Deoxyribonucleotides - metabolism
Deoxyribonucleotides - pharmacology
Leukemia, Experimental - drug therapy
Leukemia, Experimental - metabolism
Leukemia, Experimental - pathology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The growth of cultured L5178Y cells is inhibited by relatively low concentrations fo deoxyadenosine in the presence of deoxycoformycin, an inhibitor of adenosine deaminase. Cell viability is reduced, presumably as a consequence of the induced state of unbalanced growth which is characterized by inhibition in DNA synthesis, accumulation of cells in G1 or early S phase, a continuation in RNA synthesis, and increasing cell volume. The intracellular concentrations of purine and pyrimidine ribonucleoside phosphates remain essentially unchanged. The significant changes in the intracellular deoxynucleoside triphosphate pools are an increase in deoxyadenosine triphosphate and a decrease in deoxycytidine triphosphate.
ISSN:0008-5472