A rapid cell-permeating turn-on probe for sensitive and selective detection of sulfite in living cells

A rapid cell-permeating probe NJUXJ-1 was introduced for sensitive and selective detection of sulfite in living cells. It generated a turn-on response to sulfite with high sensitivity (detection limit 13.0 nM) and selectivity (at a physiological level) and low toxicity. The fluorescence of the detec...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2018-09, Vol.16 (37), p.8318-8324
Main Authors: Xu, Jing, Zheng, Da-Jun, Su, Mi-Mi, Chen, Yan-Chi, Jiao, Qing-Cai, Yang, Yu-Shun, Zhu, Hai-Liang
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cells (biology)
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
Humans
Limit of Detection
Medical imaging
Permeability
Sulfite
Sulfites - metabolism
Toxicity
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Summary:A rapid cell-permeating probe NJUXJ-1 was introduced for sensitive and selective detection of sulfite in living cells. It generated a turn-on response to sulfite with high sensitivity (detection limit 13.0 nM) and selectivity (at a physiological level) and low toxicity. The fluorescence of the detecting system was steady for a wide pH range (5-8) and a long period of time (over 12 h). The most attractive point, its rapid cell-permeating ability, made it suitable for bioimaging with a 2 min incubation time and shortened the whole detecting period (cell-permeation and reaction), and thus could decrease background interference. It offered a convenient approach for determining exogenous or endogenous sulfite levels in living cells and further applications. A rapid cell-permeating probe NJUXJ-1 was introduced for sensitive and selective detection of sulfite in living cells.
ISSN:1477-0520
1477-0539
DOI:10.1039/c8ob01908h