On-line coupling between capillary electrophoresis and microscale thermophoresis (CE-MST); the proof-of-concept

We demonstrate that microscale thermophoresis can be easily coupled with capillary electrophoresis in an on-line flow system (CE-MST), offering new potential possibilities. It takes advantage of sample separation and miniaturization prior to thermo-optical/MST analysis. No instrument modification is...

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Bibliographic Details
Published in:Analyst (London) 2018-10, Vol.143 (2), p.4854-4859
Main Authors: Nowak, Pawe M, Wo niakiewicz, Micha
Format: Article
Language:English
Subjects:
Capillary electrophoresis
Electrophoresis
Miniaturization
On-line systems
Sampling methods
Thermophoresis
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Summary:We demonstrate that microscale thermophoresis can be easily coupled with capillary electrophoresis in an on-line flow system (CE-MST), offering new potential possibilities. It takes advantage of sample separation and miniaturization prior to thermo-optical/MST analysis. No instrument modification is required. The future perspective is discussed. Capillary electrophoresis coupled on-line with microscale thermophoresis (CE-MST) may become a new method of choice for characterizing biomolecules and their interactions.
ISSN:0003-2654
1364-5528
DOI:10.1039/c8an01191e