C-terminus of Hsc70-interacting protein (CHIP) enhances stemness properties of human Wharton's jelly mesenchymal stem cell

Mesenchymal stem cells are an attractive source of multipotent cells in part because they are easy to obtain. Several E3 ligases regulate the stability and functions of various factors in different adult stem cells through the ubiquitylation pathway. We investigated the C-terminus of Hsc70-interacti...

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Bibliographic Details
Published in:Biotechnic & histochemistry 2018-11, Vol.93 (8), p.632-639
Main Authors: Yang, C-K, Feng, C-C, Lo, J-F, Chen, J-W, Padma, V. V, Lai, C-H, Chen, T-S, Chen, R-J, Liao, P-H, Huang, C-Y
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Movement
Cells, Cultured
HSC70 Heat-Shock Proteins - genetics
Hsc70-interacting protein
human
Humans
mesenchymal stem cell
Mesenchymal Stem Cells
phosphatase and tensin homolog (PTEN)
protein kinase B (Akt)
Proto-Oncogene Proteins c-akt - metabolism
self-renewal
Signal Transduction
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
Wharton Jelly
Wharton's jelly
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Summary:Mesenchymal stem cells are an attractive source of multipotent cells in part because they are easy to obtain. Several E3 ligases regulate the stability and functions of various factors in different adult stem cells through the ubiquitylation pathway. We investigated the C-terminus of Hsc70-interacting protein (CHIP) E3 ligase that regulates pluripotency of human Wharton's jelly mesenchymal stem cells (hWJMSC). We found that CHIP increases protein kinase B (Akt) phosphorylation by decreased expression of phosphatase and tensin homolog (PTEN), which suggests improvement of the survival pathway by CHIP over-expression. We also found that increased CHIP expression induced Sox2 and NANOG, which can promote stem cell self-renewal and prevent oxidative stress-induced senescence of hWJMSC by decreased p21. We found that CHIP could be used to enhance the multiple functions of hWJMSC.
ISSN:1052-0295
1473-7760
DOI:10.1080/10520295.2018.1521990