Anti-inflammatory effects of Kaempferol on Helicobacter pylori-induced inflammation

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2019-01, Vol.83 (1), p.166-173
• Main Authors: Yeon, Min Ji, Lee, Min Ho, Kim, Do Hyun, Yang, Ji Yeong, Woo, Hyun Jun, Kwon, Hye Jin, Moon, Cheol, Kim, Sa-Hyun, Kim, Jong-Bae
• Format: Article
• Language: English
• Subjects: CagA; Helicobacter pylori; kaempferol; pro-inflammatory cytokines; VacA
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2018.1528140

Inflammation induced by Helicobacter pylori infection related to gastric carcinogenesis. In this study, we have investigated the anti-inflammatory effect and its mechanism of kaempferol in the inflammatory response caused by H. pylori infection in vitro. We found that kaempferol reduced the expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-8) and production of IL-8 in AGS cells. In addition, kaempferol suppressed translocation of cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA) of H. pylori to AGS cells. It was due to decreased transcription of type IV secretion system (T4SS) components involved in CagA injection and secretion system subunit protein A (SecA) of type V secretion system (T5SS) involved in VacA secretion by kaempferol. In conclusion, kaempferol shows the anti-inflammatory effect by suppressing the translocation of CagA and VacA proteins and leading to the down-regulation of pro-inflammatory cytokines. Abbreviations: CagA: cytotoxin-associated gene A; VacA: vacuolating cytotoxin A; T4SS: type IV secretion systems; SecA: secretion system subunit protein A; T5SS: type V secretion system; Kaempferol has an anti-inflammatory effect on H. pylori infection by suppressing the translocation of CagA and VacA proteins.