Polyadenylic Acid Metabolizing Enzyme Levels During Induction of Differentiation in a Human Leukemia T-Cell Line With Phorbol Ester

Induction of differentiation of MOLT3 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) was found to affect the activity levels of the polyadenylic acid [poly(A)] metabolizing enzymes. TPA administration at a concentration of 16 nM resulted in an increase of poly(A) exonuclease and poly(A) polym...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1987-03, Vol.78 (3), p.407-411
Main Authors: Perez, Sonia, Trangas, Theoni, Kokkinopoulos, Dimitris, Tsiapalis, Chris M., Papamichail, Michael
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
Cell Differentiation - drug effects
Cell Line
Deoxyadenosines - pharmacology
Hematologic and hematopoietic diseases
Humans
Leukemia, Lymphoid - enzymology
Leukemia, Lymphoid - pathology
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Nucleotidyltransferases - metabolism
Polynucleotide Adenylyltransferase - metabolism
Ribonucleases - metabolism
T-Lymphocytes - enzymology
T-Lymphocytes - pathology
Tetradecanoylphorbol Acetate - pharmacology
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Summary:Induction of differentiation of MOLT3 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) was found to affect the activity levels of the polyadenylic acid [poly(A)] metabolizing enzymes. TPA administration at a concentration of 16 nM resulted in an increase of poly(A) exonuclease and poly(A) polymerase activities after 2 and 2.5 hours, respectively, and in a decline to control levels therafter. Cordycepin, an inhibitor of poly(A) polymerization, prevented the increasing binding of OKT11A monoclonal antibody induced by TPA treatment, whereas TPA-induced reduction in OKT6 monoclonal antibody binding persisted. The alterations in the poly(A) metabolizing enzyme activities following treatment of the cells with TPA, as well as the inhibitory effect of cordycepin, may suggest that these enzymes may be involved in the regulation of the differentiation process.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/78.3.407