Loading…
Modulation of cancer cells' radiation response in the presence of folate conjugated Au@Fe 2 O 3 nanocomplex as a targeted radiosensitizer
To investigate the effects of Au@Fe O core-shell nanoparticle (NP), with and without conjugation to folic acid (FA) as a targeting ligand, on radiosensitization of both cancer and healthy cells. Au@Fe O NPs were first synthesized, then modified with FA, and finally characterized. Radiation dose enha...
Saved in:
| Published in: | Clinical & translational oncology 2019-04, Vol.21 (4), p.479 |
|---|---|
| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | |
| container_end_page | |
| container_issue | 4 |
| container_start_page | 479 |
| container_title | Clinical & translational oncology |
| container_volume | 21 |
| creator | Mirrahimi, M Hosseini, V Shakeri-Zadeh, A Alamzadeh, Z Kamrava, S K Attaran, N Abed, Z Ghaznavi, H Hosseini Nami, S M A |
| description | To investigate the effects of Au@Fe
O
core-shell nanoparticle (NP), with and without conjugation to folic acid (FA) as a targeting ligand, on radiosensitization of both cancer and healthy cells.
Au@Fe
O
NPs were first synthesized, then modified with FA, and finally characterized. Radiation dose enhancement studies were performed on KB cancer cells and L929 healthy cells. NPs at the concentration of 20 µg/ml were first incubated with both cell lines and then different doses of 6 MV X-ray radiation were examined. The end effects were evaluated via MTT assay and flow cytometry using AnnexinV/PI kit.
It was indicated that viability of KB cells has a much lower rate than L929 cells when the cells were treated by {(FA-Au@Fe
O
) + (X-ray)} regimen. Cell viability was even decreased significantly when X-ray dose increased. Moreover, flow cytometry studies revealed that FA-targeted NPs induced higher level of apoptosis for KB cancer cells than L929 healthy cells.
Our findings provide a new perspective on high ability of the synthesized FA-targeted Au@Fe
O
NPs which may be considered as an efficient radiosensitizer in the process of targeted radiation therapy of cancer. |
| doi_str_mv | 10.1007/s12094-018-1947-8 |
| format | article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_30298468</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>30298468</sourcerecordid><originalsourceid>FETCH-LOGICAL-p93t-92174d189dc562cf0960e3bf7e54337969daa158fcfbf87384bc30a3e62b92403</originalsourceid><addsrcrecordid>eNo1kM1OwzAQhC0kREvhAbigvXEy-C-OfaOqKCAV9dJ75TjrkiqNoziRgDfgrZuqcJrR7Mx3WELuOHvkjOVPiQtmFWXcUG5VTs0FmXJtLZUsyybkOqU9G53m_IpMJBPWKG2m5PcjlkPt-io2EAN413jswGNdpwfoXFmdTx2mNjYJoWqg_0RoxwDH6mkT4rhH8LHZD7vRlTAfnpcIAtYgoXFN9PHQ1vgFLoGD3nU7PLVO9DhSUtVXP9jdkMvg6oS3fzojm-XLZvFGV-vX98V8RVsre2oFz1XJjS19poUPzGqGsgg5ZkrK3GpbOsczE3wogsmlUYWXzEnUorBCMTkj92dsOxQHLLdtVx1c9739f4k8Ai_CY0E</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Modulation of cancer cells' radiation response in the presence of folate conjugated Au@Fe 2 O 3 nanocomplex as a targeted radiosensitizer</title><source>Springer Link</source><creator>Mirrahimi, M ; Hosseini, V ; Shakeri-Zadeh, A ; Alamzadeh, Z ; Kamrava, S K ; Attaran, N ; Abed, Z ; Ghaznavi, H ; Hosseini Nami, S M A</creator><creatorcontrib>Mirrahimi, M ; Hosseini, V ; Shakeri-Zadeh, A ; Alamzadeh, Z ; Kamrava, S K ; Attaran, N ; Abed, Z ; Ghaznavi, H ; Hosseini Nami, S M A</creatorcontrib><description>To investigate the effects of Au@Fe
O
core-shell nanoparticle (NP), with and without conjugation to folic acid (FA) as a targeting ligand, on radiosensitization of both cancer and healthy cells.
Au@Fe
O
NPs were first synthesized, then modified with FA, and finally characterized. Radiation dose enhancement studies were performed on KB cancer cells and L929 healthy cells. NPs at the concentration of 20 µg/ml were first incubated with both cell lines and then different doses of 6 MV X-ray radiation were examined. The end effects were evaluated via MTT assay and flow cytometry using AnnexinV/PI kit.
It was indicated that viability of KB cells has a much lower rate than L929 cells when the cells were treated by {(FA-Au@Fe
O
) + (X-ray)} regimen. Cell viability was even decreased significantly when X-ray dose increased. Moreover, flow cytometry studies revealed that FA-targeted NPs induced higher level of apoptosis for KB cancer cells than L929 healthy cells.
Our findings provide a new perspective on high ability of the synthesized FA-targeted Au@Fe
O
NPs which may be considered as an efficient radiosensitizer in the process of targeted radiation therapy of cancer.</description><identifier>EISSN: 1699-3055</identifier><identifier>DOI: 10.1007/s12094-018-1947-8</identifier><identifier>PMID: 30298468</identifier><language>eng</language><publisher>Italy</publisher><subject>Animals ; Apoptosis - drug effects ; Apoptosis - radiation effects ; Cell Survival - drug effects ; Cell Survival - radiation effects ; Drug Delivery Systems ; Folic Acid - chemistry ; Gold - chemistry ; Humans ; KB Cells ; L Cells (Cell Line) ; Magnetite Nanoparticles - chemistry ; Mice ; Radiation Dosage ; Radiation-Sensitizing Agents - chemistry ; Radiation-Sensitizing Agents - pharmacology ; Radiation-Sensitizing Agents - toxicity ; Radiotherapy ; X-Rays</subject><ispartof>Clinical & translational oncology, 2019-04, Vol.21 (4), p.479</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-2847-9223 ; 0000-0002-4629-1697</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30298468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mirrahimi, M</creatorcontrib><creatorcontrib>Hosseini, V</creatorcontrib><creatorcontrib>Shakeri-Zadeh, A</creatorcontrib><creatorcontrib>Alamzadeh, Z</creatorcontrib><creatorcontrib>Kamrava, S K</creatorcontrib><creatorcontrib>Attaran, N</creatorcontrib><creatorcontrib>Abed, Z</creatorcontrib><creatorcontrib>Ghaznavi, H</creatorcontrib><creatorcontrib>Hosseini Nami, S M A</creatorcontrib><title>Modulation of cancer cells' radiation response in the presence of folate conjugated Au@Fe 2 O 3 nanocomplex as a targeted radiosensitizer</title><title>Clinical & translational oncology</title><addtitle>Clin Transl Oncol</addtitle><description>To investigate the effects of Au@Fe
O
core-shell nanoparticle (NP), with and without conjugation to folic acid (FA) as a targeting ligand, on radiosensitization of both cancer and healthy cells.
Au@Fe
O
NPs were first synthesized, then modified with FA, and finally characterized. Radiation dose enhancement studies were performed on KB cancer cells and L929 healthy cells. NPs at the concentration of 20 µg/ml were first incubated with both cell lines and then different doses of 6 MV X-ray radiation were examined. The end effects were evaluated via MTT assay and flow cytometry using AnnexinV/PI kit.
It was indicated that viability of KB cells has a much lower rate than L929 cells when the cells were treated by {(FA-Au@Fe
O
) + (X-ray)} regimen. Cell viability was even decreased significantly when X-ray dose increased. Moreover, flow cytometry studies revealed that FA-targeted NPs induced higher level of apoptosis for KB cancer cells than L929 healthy cells.
Our findings provide a new perspective on high ability of the synthesized FA-targeted Au@Fe
O
NPs which may be considered as an efficient radiosensitizer in the process of targeted radiation therapy of cancer.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - radiation effects</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>Drug Delivery Systems</subject><subject>Folic Acid - chemistry</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>KB Cells</subject><subject>L Cells (Cell Line)</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Mice</subject><subject>Radiation Dosage</subject><subject>Radiation-Sensitizing Agents - chemistry</subject><subject>Radiation-Sensitizing Agents - pharmacology</subject><subject>Radiation-Sensitizing Agents - toxicity</subject><subject>Radiotherapy</subject><subject>X-Rays</subject><issn>1699-3055</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo1kM1OwzAQhC0kREvhAbigvXEy-C-OfaOqKCAV9dJ75TjrkiqNoziRgDfgrZuqcJrR7Mx3WELuOHvkjOVPiQtmFWXcUG5VTs0FmXJtLZUsyybkOqU9G53m_IpMJBPWKG2m5PcjlkPt-io2EAN413jswGNdpwfoXFmdTx2mNjYJoWqg_0RoxwDH6mkT4rhH8LHZD7vRlTAfnpcIAtYgoXFN9PHQ1vgFLoGD3nU7PLVO9DhSUtVXP9jdkMvg6oS3fzojm-XLZvFGV-vX98V8RVsre2oFz1XJjS19poUPzGqGsgg5ZkrK3GpbOsczE3wogsmlUYWXzEnUorBCMTkj92dsOxQHLLdtVx1c9739f4k8Ai_CY0E</recordid><startdate>20190409</startdate><enddate>20190409</enddate><creator>Mirrahimi, M</creator><creator>Hosseini, V</creator><creator>Shakeri-Zadeh, A</creator><creator>Alamzadeh, Z</creator><creator>Kamrava, S K</creator><creator>Attaran, N</creator><creator>Abed, Z</creator><creator>Ghaznavi, H</creator><creator>Hosseini Nami, S M A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><orcidid>https://orcid.org/0000-0002-2847-9223</orcidid><orcidid>https://orcid.org/0000-0002-4629-1697</orcidid></search><sort><creationdate>20190409</creationdate><title>Modulation of cancer cells' radiation response in the presence of folate conjugated Au@Fe 2 O 3 nanocomplex as a targeted radiosensitizer</title><author>Mirrahimi, M ; Hosseini, V ; Shakeri-Zadeh, A ; Alamzadeh, Z ; Kamrava, S K ; Attaran, N ; Abed, Z ; Ghaznavi, H ; Hosseini Nami, S M A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p93t-92174d189dc562cf0960e3bf7e54337969daa158fcfbf87384bc30a3e62b92403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - radiation effects</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - radiation effects</topic><topic>Drug Delivery Systems</topic><topic>Folic Acid - chemistry</topic><topic>Gold - chemistry</topic><topic>Humans</topic><topic>KB Cells</topic><topic>L Cells (Cell Line)</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Mice</topic><topic>Radiation Dosage</topic><topic>Radiation-Sensitizing Agents - chemistry</topic><topic>Radiation-Sensitizing Agents - pharmacology</topic><topic>Radiation-Sensitizing Agents - toxicity</topic><topic>Radiotherapy</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mirrahimi, M</creatorcontrib><creatorcontrib>Hosseini, V</creatorcontrib><creatorcontrib>Shakeri-Zadeh, A</creatorcontrib><creatorcontrib>Alamzadeh, Z</creatorcontrib><creatorcontrib>Kamrava, S K</creatorcontrib><creatorcontrib>Attaran, N</creatorcontrib><creatorcontrib>Abed, Z</creatorcontrib><creatorcontrib>Ghaznavi, H</creatorcontrib><creatorcontrib>Hosseini Nami, S M A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Clinical & translational oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mirrahimi, M</au><au>Hosseini, V</au><au>Shakeri-Zadeh, A</au><au>Alamzadeh, Z</au><au>Kamrava, S K</au><au>Attaran, N</au><au>Abed, Z</au><au>Ghaznavi, H</au><au>Hosseini Nami, S M A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of cancer cells' radiation response in the presence of folate conjugated Au@Fe 2 O 3 nanocomplex as a targeted radiosensitizer</atitle><jtitle>Clinical & translational oncology</jtitle><addtitle>Clin Transl Oncol</addtitle><date>2019-04-09</date><risdate>2019</risdate><volume>21</volume><issue>4</issue><spage>479</spage><pages>479-</pages><eissn>1699-3055</eissn><abstract>To investigate the effects of Au@Fe
O
core-shell nanoparticle (NP), with and without conjugation to folic acid (FA) as a targeting ligand, on radiosensitization of both cancer and healthy cells.
Au@Fe
O
NPs were first synthesized, then modified with FA, and finally characterized. Radiation dose enhancement studies were performed on KB cancer cells and L929 healthy cells. NPs at the concentration of 20 µg/ml were first incubated with both cell lines and then different doses of 6 MV X-ray radiation were examined. The end effects were evaluated via MTT assay and flow cytometry using AnnexinV/PI kit.
It was indicated that viability of KB cells has a much lower rate than L929 cells when the cells were treated by {(FA-Au@Fe
O
) + (X-ray)} regimen. Cell viability was even decreased significantly when X-ray dose increased. Moreover, flow cytometry studies revealed that FA-targeted NPs induced higher level of apoptosis for KB cancer cells than L929 healthy cells.
Our findings provide a new perspective on high ability of the synthesized FA-targeted Au@Fe
O
NPs which may be considered as an efficient radiosensitizer in the process of targeted radiation therapy of cancer.</abstract><cop>Italy</cop><pmid>30298468</pmid><doi>10.1007/s12094-018-1947-8</doi><orcidid>https://orcid.org/0000-0002-2847-9223</orcidid><orcidid>https://orcid.org/0000-0002-4629-1697</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1699-3055 |
| ispartof | Clinical & translational oncology, 2019-04, Vol.21 (4), p.479 |
| issn | 1699-3055 |
| language | eng |
| recordid | cdi_pubmed_primary_30298468 |
| source | Springer Link |
| subjects | Animals Apoptosis - drug effects Apoptosis - radiation effects Cell Survival - drug effects Cell Survival - radiation effects Drug Delivery Systems Folic Acid - chemistry Gold - chemistry Humans KB Cells L Cells (Cell Line) Magnetite Nanoparticles - chemistry Mice Radiation Dosage Radiation-Sensitizing Agents - chemistry Radiation-Sensitizing Agents - pharmacology Radiation-Sensitizing Agents - toxicity Radiotherapy X-Rays |
| title | Modulation of cancer cells' radiation response in the presence of folate conjugated Au@Fe 2 O 3 nanocomplex as a targeted radiosensitizer |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T03%3A13%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Modulation%20of%20cancer%20cells'%20radiation%20response%20in%20the%20presence%20of%20folate%20conjugated%20Au@Fe%202%20O%203%20nanocomplex%20as%20a%20targeted%20radiosensitizer&rft.jtitle=Clinical%20&%20translational%20oncology&rft.au=Mirrahimi,%20M&rft.date=2019-04-09&rft.volume=21&rft.issue=4&rft.spage=479&rft.pages=479-&rft.eissn=1699-3055&rft_id=info:doi/10.1007/s12094-018-1947-8&rft_dat=%3Cpubmed%3E30298468%3C/pubmed%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p93t-92174d189dc562cf0960e3bf7e54337969daa158fcfbf87384bc30a3e62b92403%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/30298468&rfr_iscdi=true |