Effects of melatonin on acetylsalicylic acid induced gastroduodenal and jejunal mucosal injury

• Published in: Biotechnic & histochemistry 2018-10, Vol.93 (7), p.485-495
• Main Authors: Taslidere, E, Vardi, N, Parlakpinar, H, Yıldız, A, Taslidere, B, Karaaslan, MG
• Format: Article
• Language: English
• Subjects: acetylsalicylic acid; Animals; aspirin; Aspirin - toxicity; duodenum; Gastric Mucosa - drug effects; Gastric Mucosa - injuries; Gastric Mucosa - pathology; Injections, Intraperitoneal; jejunum; Jejunum - injuries; Jejunum - pathology; Male; melatonin; Melatonin - administration & dosage; Melatonin - pharmacology; rat; Rats; Rats, Wistar; Reference Standards; stomach
• ISSN: 1052-0295; 1473-7760
• DOI: 10.1080/10520295.2018.1442020

We evaluated the effects of melatonin on acetylsalicylic acid (ASA) induced gastroduodenal and jejunal mucosal injury. We used 40 postpubertal rats divided randomly into five groups of eight animals. The control group consisted of untreated animals. The Mel group was injected intraperitoneally (i.p.) with 5 mg/kg melatonin. The ASA group was injected i.p. with 200 mg/kg ASA. The ASA + Mel group was injected i.p. with 5 mg/kg melatonin 45 min after administering 200 mg/kg ASA i.p. The Mel + ASA group was injected i.p. with 5 mg/kg melatonin 45 min before administering 200 mg/kg ASA i.p. We found no statistically significant differences in mean histopathological scores in the ASA + Mel group compared to the ASA group. ASA caused shortened villi and loss of the apical villus in the duodenum. The histopathological score was increased and villus height was decreased in the ASA group compared to untreated controls. Treatment with melatonin attenuated the histological damage. In the ASA group, occasional areas showed erosion of villi in the jejunum; however, differences in mean histopathological score in ASA group compared to the other groups were not statistically significant. Malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) activities were measured in stomach, duodenal and jejunum tissue. We found increased MDA activity in both stomach and duodenal tissues in the ASA group compared to the control group (p