The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C . e legans

Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeosta...

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Bibliographic Details
Published in:eLife 2018-12, Vol.7
Main Authors: Davis, Gregory M, Tu, Shikui, Anderson, Joshua Wt, Colson, Rhys N, Gunzburg, Menachem J, Francisco, Michelle A, Ray, Debashish, Shrubsole, Sean P, Sobotka, Julia A, Seroussi, Uri, Lao, Robert X, Maity, Tuhin, Wu, Monica Z, McJunkin, Katherine, Morris, Quaid D, Hughes, Timothy R, Wilce, Jacqueline A, Claycomb, Julie M, Weng, Zhiping, Boag, Peter R
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans - metabolism
Caenorhabditis elegans Proteins - metabolism
Carrier Proteins - metabolism
Chromatin - metabolism
Gene Regulatory Networks
Germ Cells - metabolism
RNA Interference
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Summary:Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
ISSN:2050-084X