Elucidating the chemical and biochemical applications of Citrus sinensis-mediated silver nanocrystal

Synthesis of nanoparticles using biodegradable source is safer and echo-friendly. Here, we describe the synthesis of polycrystalline silver nanocrystals using Citrus sinensis acting as both reducing and capping agents. After exposing the silver ions to orange extract, rapid reduction of silver ions...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomolecular structure & dynamics 2019-12, Vol.37 (18), p.4863-4874
Main Authors: Siddiqui, Nurunnesa, Masum, Abdulla Al, Uddin, Md. Raihan, Mandal, Sukhendu, Sengupta, Mohana, Islam, Md. Maidul, Mukhopadhyay, Subrata
Format: Article
Language:English
Subjects:
antibacterial activity
catalytic activity
cytotoxicity study
Green synthesis
mercury sensing
orange extract (Citrus sinensis)
protein binding
silver nanocrystals
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Synthesis of nanoparticles using biodegradable source is safer and echo-friendly. Here, we describe the synthesis of polycrystalline silver nanocrystals using Citrus sinensis acting as both reducing and capping agents. After exposing the silver ions to orange extract, rapid reduction of silver ions led to the formation of stable silver nanocrystals due to the reducing and stabilizing properties of orange fruit juice. The synthesized silver nanocrystals were characterized using various analytical techniques like UV-vis spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscope (SEM) and transmission electron microscopy (TEM). The biochemical activity of the synthesized nanocrystals was studied in the light of affinity to bovine serum albumin using several biophysical methods like absorbance, fluorescence and circular dichroism spectroscopy. Cytotoxic activity of these nanocrystals was also studied against Hep-2 cell line using fluorescence microscopy. It was also found that the synthesized nanocrystals can sense mercuric ion down to 50 µM in the presence of a number of cations. Furthermore, we established that the silver nanoparticles can effectively catalyse the reduction of methylene blue by ascorbic acid. The present study will enrich our knowledge on the chemical and biochemical activities of green-synthesized silver nanocrystals.
ISSN:0739-1102
1538-0254
DOI:10.1080/07391102.2018.1559763