GR CALUX assay detects synthetic glucocorticoids in calf urine: a validation study

Member States of the EU are required to monitor the use of pharmacologically active substances in food-producing animals. There is evidence, however, that the target-based approach currently applied in official monitoring plans might under-estimate the real incidence of growth promoter abuse in live...

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Published in:Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2019-03, Vol.36 (3), p.413-423
Main Authors: Pitardi, Danilo, Meloni, Daniela, Olivo, Fabio, Loprevite, Daniela, Cavarretta, Maria C., Behnisch, Peter, Brouwer, Abraham, Felzel, Emiel, Ingravalle, Francesco, Capra, Pierluigi, Gili, Marilena, Pezzolato, Marzia, Bozzetta, Elena
Format: Article
Language:English
Subjects:
Abuse
Analytical chemistry
Animals
Assaying
Biological activity
Biological Assay
Biological effects
calves
Cattle
Chromatography, Liquid
Dexamethasone
Drug abuse
Glucocorticoids
Glucocorticoids - chemical synthesis
Glucocorticoids - metabolism
Glucocorticoids - urine
Hormones
illicit treatment
Livestock
Luciferases - genetics
Luciferases - metabolism
Methylprednisolone
Organic chemistry
Pharmacology
Prednisolone
Qualitative analysis
reporter gene assay
Ruggedness
Sex hormones
Spectrometry, Mass, Electrospray Ionization
Urine
urine samples
Validation
Veterinary medicine
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Summary:Member States of the EU are required to monitor the use of pharmacologically active substances in food-producing animals. There is evidence, however, that the target-based approach currently applied in official monitoring plans might under-estimate the real incidence of growth promoter abuse in livestock. As demonstrated for sex hormones, the association of effect-oriented biological screening with chemical confirmatory techniques could be the best strategy in revealing the abuse of veterinary drugs. Here we demonstrate the reliability of a cell-based assay to screen calf urine samples for synthetic glucocorticoids. The validation included the most widely used synthetic drugs (flumethasone, dexamethasone, betamethasone, methylprednisolone and prednisolone) and was developed according to the Commission Decision 2002/657/EC, thus including the verification of cut-off level, the β error, the specificity, ruggedness and stability. The study was carried out using prednisolone as representative substance at 5 ng mL −1 concentration. All blank and spiked urine fulfilled the EU criteria, moreover the method resulted in being specific and sound, and the analytes in urine were stable for at least 30 days. The assay results indicated its suitability for a qualitative analysis of calf urine samples. This method enabled the detection of low doses of synthetic glucocorticoids (GCs) in matrix (
ISSN:1944-0049
1944-0057
DOI:10.1080/19440049.2019.1568584