A β 2 -tubulin dsRNA derived from Fusarium asiaticum confers plant resistance to multiple phytopathogens and reduces fungicide resistance

Crops are attacked by a large number of pathogens which are responsible for an approximately 30% loss in global crop production at pre- and post-harvest levels. In light of the continuing emergence of fungicide resistance, the needs for new agricultural drugs turn out to be much more critical. Here...

Full description

Saved in:
Bibliographic Details
Published in:Pesticide biochemistry and physiology 2019-01, Vol.153, p.36
Main Authors: Gu, Kai-Xin, Song, Xiu-Shi, Xiao, Xue-Mei, Duan, Xiao-Xin, Wang, Jian-Xin, Duan, Ya-Bing, Hou, Yi-Ping, Zhou, Ming-Guo
Format: Article
Language:English
Subjects:
Disease Resistance
Drug Resistance, Fungal - genetics
Fungal Proteins - genetics
Fungicides, Industrial - toxicity
Fusarium - genetics
Fusarium - pathogenicity
RNA, Double-Stranded - genetics
RNA, Fungal - genetics
Triticum - microbiology
Tubulin - genetics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Crops are attacked by a large number of pathogens which are responsible for an approximately 30% loss in global crop production at pre- and post-harvest levels. In light of the continuing emergence of fungicide resistance, the needs for new agricultural drugs turn out to be much more critical. Here we demonstrated a Faβ Tub-3 dsRNA derived from Fusarium asiaticum had broad-spectrum antifungal activity against Fusarium spp., Botrytis cinerea, Magnaporthe oryzae and Colletotrichum truncatum, with an additional function of reducing the dosage of carbendazim (MBC) fungicide. RNAi molecules derived from different regions of β -tubulin gene had different effects on mycelial growth, asexual reproduction and virulence. Faβ Tub-3 (one of β -tubulin segments) exhibited a strong silencing efficacy both on β -tubulin and β -tubulin genes in F. asiaticum. Faβ Tub-3 sequence was found to be highly conserved among Fusarium spp., Botrytis cinerea, Magnaporthe oryzae and Colletotrichum truncatum. The Faβ Tub-3 dsRNA demonstrated a broad-spectrum antifungal activity against these fungi in vitro and on living plant. More importantly, Faβ Tub-3 dsRNA increased the fungal sensitivity to MBC, while MBC increased the duration of Faβ Tub-3 dsRNA. Our findings suggest a new anti-fungal agent (Faβ Tub-3 dsRNA) for plant protection against diverse pathogens and for fungicide reduction.
ISSN:1095-9939
DOI:10.1016/j.pestbp.2018.10.005