Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus

RNA transcripts of cloned cDNA of the genomic RNAs of BBV (black beetle virus) are infectious to cultured cells of Drosophila melanogaster. Individual transcripts had approximately 10% of the infectivity of the corresponding authentic virion RNA. Progeny virus resulting from transcript infection was...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-01, Vol.83 (1), p.63-66
Main Authors: Dasmahapatra, B, Dasgupta, R, Saunders, K, Selling, B, Gallagher, T, Kaesberg, P
Format: Article
Language:English
Subjects:
ADN
Animals
ARN
Biological and medical sciences
Biotechnology
Centrifugation, Density Gradient
Complementary DNA
Cultured cells
DNA
DNA - genetics
DNA, Recombinant
DROSOPHILA
DROSOPHILA MELANOGASTER
Drosophila melanogaster - genetics
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetic engineering
Genetic technics
Genomics
INFECTIVITY
Insect Viruses - genetics
INVERTEBRATE VIRUSES
Methods. Procedures. Technologies
nodaviridae
PODER INFECCIOSO
POUVOIR INFECTANT
RNA
RNA, Viral - genetics
Transcription, Genetic
Transfection
Viral morphology
Viral Plaque Assay
Viral RNA
Virion - genetics
Virions
VIRUS DE INVERTEBRADOS
VIRUS DES INVERTEBRES
Viruses
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Description
Summary:RNA transcripts of cloned cDNA of the genomic RNAs of BBV (black beetle virus) are infectious to cultured cells of Drosophila melanogaster. Individual transcripts had approximately 10% of the infectivity of the corresponding authentic virion RNA. Progeny virus resulting from transcript infection was phenotypically indistinguishable from the progenitor virus used to generate the original cDNA forms as judged by sucrose density gradient sedimentation, specific infectivity, plaque morphology, and serology. Although the transcript RNAs used to produce this virus had 20 nonviral bases headed by a capping group at their 5′termini, these 20 bases were absent in the progeny viral RNAs. The cDNA forms, and therefore the resulting transcript RNAs, should be readily modifiable by the techniques of recombinant DNA technology both for viral studies and for the insertion of foreign genes into the viral genome and thus into the host cytoplasm.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.1.63