Bioactivity-based analysis and chemical characterization of cytotoxic compounds from a poisonous mushroom, Amanita spissacea, in human lung cancer cells in vitro

As part of our systematic study on Korean toxic mushrooms, bioactivity-guided fractionation of the MeOH extract of Amanita spissacea (Amanitaceae) fruiting bodies and chemical investigation of its cytotoxic fractions led to the isolation of (9E)-8-oxo-9-octadecenoic acid (1), (10E)-9-oxo-10-octadece...

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Bibliographic Details
Published in:Natural product research 2021-02, Vol.35 (4), p.649-654
Main Authors: So, Hae Min, Lee, Seul, Baek, Kwan-Hyuck, Roh, Hyun-Soo, Kim, Sil, Jo, Mun Seok, Baek, Su Cheol, Seok, Soonja, Ryoo, Rhim, Kim, Ki Hyun
Format: Article
Language:English
Subjects:
Acids
Amanita - chemistry
Amanita spissacea
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Biocompatibility
Biological activity
Caspase-3
Cell Line, Tumor
Cell Shape - drug effects
Chemical compounds
Chromatography, High Pressure Liquid
Cytotoxicity
Drug development
fatty acids
Fractionation
Fruit bodies
Humans
Lung cancer
Lung Neoplasms - pathology
Methanol
Mushrooms
NMR
Nuclear magnetic resonance
Palmitic acid
Plant Extracts - pharmacology
poisonous mushroom
Toxicity
Tumor cell lines
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Summary:As part of our systematic study on Korean toxic mushrooms, bioactivity-guided fractionation of the MeOH extract of Amanita spissacea (Amanitaceae) fruiting bodies and chemical investigation of its cytotoxic fractions led to the isolation of (9E)-8-oxo-9-octadecenoic acid (1), (10E)-9-oxo-10-octadecenoic acid (2), (9E)-8-oxo-9-octadecenoate methyl ester (3), (9Z)-9-octadecenoate-(2'S)-2',3'-dihydroxypropyl ester (4), (9Z)-9-octadecenoic acid (5), and palmitic acid (6). The structures of the isolates were elucidated by NMR spectroscopic analysis and LC/MS analysis. Among the isolated compounds, compounds 1 and 2 exhibited the most potent cytotoxic activity in all human lung cancer cell lines examined, with IC 50 values ranging from 255.7 to 321.0 μM and 250.2 to 322.5 μM, respectively. The cytotoxicity of these compounds was also found to be mediated by apoptosis associated with caspase-3 activation. These findings provide experimental evidence suggesting the potential of A. spissacea as a promising natural source for the discovery of novel anticancer drug candidates.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2019.1586699