Application of ELISA to Retail Survey of Aflatoxin B 1 in Peanut Butter

A simple procedure was devised for the routine screening of aflatoxin B (AFB ) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract w...

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Bibliographic Details
Published in:Journal of food protection 1986-10, Vol.49 (10), p.792
Main Authors: Ram, Bhanu P, Hart, L Patrick, Cole, Richard J, Pestka, James J
Format: Article
Language:English
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Summary:A simple procedure was devised for the routine screening of aflatoxin B (AFB ) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract was filtered and aqueous filtrate analyzed by a direct competitive ELISA. Recovery of AFB added to peanut butter samples ranged from 85 to 112%, with an average inter-well coefficient of variation of 18.4%. The inter-assay coefficient of variation was 22.7%. Using this procedure, only 3 of 63 commercial samples of peanut butter had detectable levels (>5.0 μg/kg) of AFB .
ISSN:1944-9097
DOI:10.4315/0362-028X-49.10.792