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Application of ELISA to Retail Survey of Aflatoxin B 1 in Peanut Butter
A simple procedure was devised for the routine screening of aflatoxin B (AFB ) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract w...
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| Published in: | Journal of food protection 1986-10, Vol.49 (10), p.792 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| container_issue | 10 |
| container_start_page | 792 |
| container_title | Journal of food protection |
| container_volume | 49 |
| creator | Ram, Bhanu P Hart, L Patrick Cole, Richard J Pestka, James J |
| description | A simple procedure was devised for the routine screening of aflatoxin B
(AFB
) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB
and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract was filtered and aqueous filtrate analyzed by a direct competitive ELISA. Recovery of AFB
added to peanut butter samples ranged from 85 to 112%, with an average inter-well coefficient of variation of 18.4%. The inter-assay coefficient of variation was 22.7%. Using this procedure, only 3 of 63 commercial samples of peanut butter had detectable levels (>5.0 μg/kg) of AFB
. |
| doi_str_mv | 10.4315/0362-028X-49.10.792 |
| format | article |
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(AFB
) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB
and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract was filtered and aqueous filtrate analyzed by a direct competitive ELISA. Recovery of AFB
added to peanut butter samples ranged from 85 to 112%, with an average inter-well coefficient of variation of 18.4%. The inter-assay coefficient of variation was 22.7%. Using this procedure, only 3 of 63 commercial samples of peanut butter had detectable levels (>5.0 μg/kg) of AFB
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(AFB
) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB
and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract was filtered and aqueous filtrate analyzed by a direct competitive ELISA. Recovery of AFB
added to peanut butter samples ranged from 85 to 112%, with an average inter-well coefficient of variation of 18.4%. The inter-assay coefficient of variation was 22.7%. Using this procedure, only 3 of 63 commercial samples of peanut butter had detectable levels (>5.0 μg/kg) of AFB
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(AFB
) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB
and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract was filtered and aqueous filtrate analyzed by a direct competitive ELISA. Recovery of AFB
added to peanut butter samples ranged from 85 to 112%, with an average inter-well coefficient of variation of 18.4%. The inter-assay coefficient of variation was 22.7%. Using this procedure, only 3 of 63 commercial samples of peanut butter had detectable levels (>5.0 μg/kg) of AFB
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| identifier | EISSN: 1944-9097 |
| ispartof | Journal of food protection, 1986-10, Vol.49 (10), p.792 |
| issn | 1944-9097 |
| language | eng |
| recordid | cdi_pubmed_primary_30959593 |
| source | ScienceDirect Journals |
| title | Application of ELISA to Retail Survey of Aflatoxin B 1 in Peanut Butter |
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