CoQ 10 production in Schizosaccharomyces pombe is increased by reduction of glucose levels or deletion of pka1

Coenzyme Q (CoQ) is an essential component of the electron transport system that produces ATP in nearly all living cells. CoQ is a popular commercial food supplement around the world, and demand for efficient production of this molecule has increased in recent years. In this study, we explored CoQ p...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2019-06, Vol.103 (12), p.4899
Main Authors: Nishida, Ikuhisa, Yokomi, Kazumasa, Hosono, Kouji, Hayashi, Kazuhiro, Matsuo, Yasuhiro, Kaino, Tomohiro, Kawamukai, Makoto
Format: Article
Language:English
Subjects:
Catalytic Domain
Culture Media - chemistry
Cyclic AMP-Dependent Protein Kinases - genetics
Cyclic AMP-Dependent Protein Kinases - metabolism
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Deletion
Glucose - chemistry
Maltose - chemistry
Schizosaccharomyces - metabolism
Signal Transduction
Ubiquinone - analogs & derivatives
Ubiquinone - biosynthesis
Ubiquinone - metabolism
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Summary:Coenzyme Q (CoQ) is an essential component of the electron transport system that produces ATP in nearly all living cells. CoQ is a popular commercial food supplement around the world, and demand for efficient production of this molecule has increased in recent years. In this study, we explored CoQ production in the fission yeast Schizosaccharomyces pombe. We found that CoQ level was higher in stationary phase than in log phase, and that it increased when the cells were grown in a low concentration of glucose, in maltose, or in glycerol/ethanol medium. Because glucose signaling is mediated by cAMP, we evaluated the involvement of this pathway in CoQ biosynthesis. Loss of Pka1, the catalytic subunit of cAMP-dependent protein kinase, increased production of CoQ , whereas loss of the regulatory subunit Cgs1 decreased production. Manipulation of other components of the cAMP-signaling pathway affected CoQ production in a consistent manner. We also found that glycerol metabolism was controlled by the cAMP/PKA pathway. CoQ production by the S. pombe ∆pka1 reached 0.98 mg/g dry cell weight in medium containing a non-fermentable carbon source [2% glycerol (w/v) and 1% ethanol (w/v) supplemented with 0.5% casamino acids (w/v)], twofold higher than the production in wild-type cells under normal growth conditions. These findings demonstrate that carbon source, growth phase, and the cAMP-signaling pathway are important factors in CoQ production in S. pombe.
ISSN:1432-0614