Effect of Elastin Peptides on Ion Fluxes in Mononuclear Cells, Fibroblasts, and Smooth Muscle Cells

• Published in: Proceedings of the National Academy of Sciences - PNAS 1987-02, Vol.84 (4), p.995-999
• Main Authors: Jacob, Marie-Paule, Fülöp, Tamàs, Foris, Gabriella, Robert, Ladislas
• Format: Article
• Language: English
• Subjects: Adult; Aorta; Biological and medical sciences; Biological Transport; Blood Cells - metabolism; Calcimycin - pharmacology; Calcium; Calcium - metabolism; Cell membranes; Cell physiology; Cells; Elastin - pharmacology; Enzymes; Fibroblasts; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Ions; Lesions; Male; Membrane and intracellular transports; Middle Aged; Molecular and cellular biology; Monocytes; Monocytes - metabolism; Muscle, Smooth - cytology; Muscle, Smooth - metabolism; Peptides - pharmacology; Potassium - metabolism; Smooth muscle myocytes; Sodium - metabolism; Trifluoperazine - pharmacology; Ungulates
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.84.4.995

Elastin peptides prepared by alcoholic potassium hydroxide degradation of highly purified fibrous elastin from bovine ligamentum nuchae (κ -elastin) were shown to act on the ion channels of human monocytes, aorta smooth muscle cells, and skin fibroblasts. In small amounts (between 0.1 and 1 μ g/ml), elastin peptides strongly increased calcium influx and inhibited calcium efflux by an apparently calmodulin-dependent mechanism. They also were shown to increase sodium influx and to decrease rubidium influx in monocyte preparations obtained from human blood. Only the ouabainsensitive portion of rubidium influx was inhibited. The action of elastin peptides is strongly concentration-dependent; the maximal activity observed in the above reactions was