On-line rapid purification of [ 13 N]N 2 gas for visualization of nitrogen fixation and translocation in nodulated soybean

Accurate analysis of N fixation in leguminous crops requires determination of N utilization within an intact plant; however, most approaches require tissue disassembly. We developed a simple and rapid technique to generate high-purity and high-yield [ N]N gas and obtained real-time images of N fixat...

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Bibliographic Details
Published in:Applied radiation and isotopes 2019-09, Vol.151, p.7
Main Authors: Yin, Yong-Gen, Ishii, Satomi, Suzui, Nobuo, Igura, Masato, Kurita, Keisuke, Miyoshi, Yuta, Nagasawa, Naotsugu, Taguchi, Mitsumasa, Kawachi, Naoki
Format: Article
Language:English
Subjects:
Biological Transport
Chromatography, Gas
Crops, Agricultural - metabolism
Glycine max - metabolism
Nitrogen - metabolism
Nitrogen Fixation
Nitrogen Radioisotopes - isolation & purification
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Summary:Accurate analysis of N fixation in leguminous crops requires determination of N utilization within an intact plant; however, most approaches require tissue disassembly. We developed a simple and rapid technique to generate high-purity and high-yield [ N]N gas and obtained real-time images of N fixation in an intact soybean plant. The purification efficiency was ∼81.6% after decay correction. Our method provides accurate signals of N fixation and allows free changes to the tracer gas composition to suit different experimental designs.
ISSN:1872-9800