Molecular characterization and antimicrobial resistance profiles of Mycobacterium tuberculosis complex in environmental substrates from three dairy farms in Eastern Cape, South Africa

This study analysed 330 environmental substrates from three dairy farms for the occurrence, drug resistance and the genetic mutations of MTBC (Mycobacterium tuberculosis complex) in Eastern Cape, South Africa using PCR, while the Genotype MTBDRplus assay was used for drug susceptibility and genetic...

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Bibliographic Details
Published in:International journal of environmental health research 2021-03, Vol.31 (2), p.215-224
Main Authors: Ntloko, Athini, Adefisoye, Martins Ajibade, Green, Ezekiel
Format: Article
Language:English
Subjects:
Animal Feed - microbiology
anti-TB drugs
Antimicrobial resistance
Bacterial Proteins - genetics
Dairy farming
Dairy farms
Dairying - standards
DNA-Directed RNA Polymerases - genetics
Drug resistance
Drug Resistance, Bacterial - drug effects
Drug Resistance, Bacterial - genetics
environment
Farms
Genes, Bacterial
Genetic analysis
Genetic diversity
Genotypes
Isoniazid
Isoniazid - pharmacology
MTBC
Multidrug resistance
Multidrug resistant organisms
Mutation
mutations
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Rifampin
RpoB protein
Soil Microbiology - standards
Soil water
South Africa
Substrates
Tuberculosis
Water Microbiology - standards
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Summary:This study analysed 330 environmental substrates from three dairy farms for the occurrence, drug resistance and the genetic mutations of MTBC (Mycobacterium tuberculosis complex) in Eastern Cape, South Africa using PCR, while the Genotype MTBDRplus assay was used for drug susceptibility and genetic mutations analyses. About 17% (55/330) of the samples were positive for MTBC at 16.7% (water), 13.3% (soil) and 20% (hayfeed). Isoniazid resistance was detected in 47.3% (26/55) of the samples while 16.4% (9/55) were multidrug-resistant. Genetic mutations were detected on the rpoB gene (resistance to rifampicin) with frequencies ranging from 53.6% (D516V) to 21.4% (H526D), while mutations on the katG and inhA genes (resistance to isoniazid) ranged between 14.3% and 80%. Incidents of diverse genetic mutations in the environmental matrices suggest possible resistance to other anti-TB drugs not assayed in this study and emphasizes the need for continuous monitoring of drug resistance patterns for timely detection and control of new clonal groups of MTBC.
ISSN:0960-3123
1369-1619
DOI:10.1080/09603123.2019.1642458