Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. The substrate stainless steel (SS) was first coated with a polydopamine layer and subsequently grafted with poly(ethylene glycol) methacrylate brushes, onto which a sandwich immunosensor w...

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Bibliographic Details
Published in:Analyst (London) 2019-10, Vol.144 (19), p.5682-569
Main Authors: Deng, Fei, Li, Yi, Hossain, Md Jakir, Kendig, Michael D, Arnold, Ria, Goldys, Ewa M, Morris, Margaret J, Liu, Guozhen
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Antibodies, Immobilized - immunology
Antibodies, Monoclonal, Murine-Derived - immunology
Biological properties
Blood
Cytokines
Equidae
Fluorescence
Fluorescent Dyes - chemistry
Goats
Immunoassay - instrumentation
Immunoassay - methods
Immunosensors
Indoles - chemistry
Interleukin-1beta - blood
Interleukin-1beta - immunology
Interleukins
Limit of Detection
Methacrylates - chemistry
Mice
Monitoring
Oxazines - chemistry
Polyethylene glycol
Polyethylene Glycols - chemistry
Polymers - chemistry
Polystyrenes - chemistry
Rats
Reproducibility of Results
Selectivity
Stainless Steel - chemistry
Stainless steels
Substrates
Target detection
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Summary:A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. The substrate stainless steel (SS) was first coated with a polydopamine layer and subsequently grafted with poly(ethylene glycol) methacrylate brushes, onto which a sandwich immunosensor was modified for detection of IL-1β. The device has been successfully applied for monitoring of IL-1β with a limit of detection of 4.7 pg mL −1 , and a linear detection range of 12.5-200 pg mL −1 . Good specificity and selectivity for monitoring of IL-1β in rat macrophage secretion were achieved. Furthermore, this device was validated by detection of IL-1β in rat whole blood samples with greater concentrations observed in obese rats compared to control, and strong positive correlation between concentrations of IL-1β and blood glucose. These results suggest this device is feasible for direct detection of target analytes in biological samples. A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood.
ISSN:0003-2654
1364-5528
DOI:10.1039/c9an01300h