MiR-92a antagonized the facilitation effect of extracellular matrix protein 1 in GC metastasis through targeting its 3'UTR region

MicroRNAs were known to play very important roles in human diseases, and have attracted great interests of research scientists in medicine, toxicology and functional foods. Gastric carcinoma (GC) remains one of the most common and lethal types of malignancy worldwide. However, the molecular mechanis...

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Bibliographic Details
Published in:Food and chemical toxicology 2019-11, Vol.133, p.110779
Main Authors: Liu, Lin-Qing, Hu, Lei, Hu, Xi-Bei, Xu, Jiang, Wu, Ai-Mei, Chen, Hao, Gu, Peng-Yin, Hu, Shi-Lian
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Animals
Carcinoma - diagnosis
Carcinoma - physiopathology
Cell Line, Tumor
Cell Movement - physiology
Cell Proliferation - physiology
Epithelial-Mesenchymal Transition - physiology
Extracellular Matrix Proteins - genetics
Extracellular Matrix Proteins - metabolism
Humans
Male
Mice, Inbred BALB C
Mice, Nude
MicroRNAs - metabolism
Neoplasm Metastasis - physiopathology
Prognosis
Stomach Neoplasms - diagnosis
Stomach Neoplasms - physiopathology
Up-Regulation
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Summary:MicroRNAs were known to play very important roles in human diseases, and have attracted great interests of research scientists in medicine, toxicology and functional foods. Gastric carcinoma (GC) remains one of the most common and lethal types of malignancy worldwide. However, the molecular mechanism of GC and the role of microRNAs in GC development remain unclear. The expression of extracellular matrix protein 1 (ECM1) is up-regulated in many cancer types, but its functional role is inconstant. In the present study, we aimed to investigate the correlation between GC development and ECM1 expression, along with its regulation by microRNAs. Immunohistochemical results showed that ECM1 was up-regulated in GC tissues and ECM1 expression level was negatively correlated with the prognosis of GC. ECM1 was found to promote gastric cancer cell metastasis in cell migration assays by facilitating the expression of proteins involved in epithelial-mesenchymal transition (EMT). MiR-92a was recognized for the first time to suppress the migration of human GC cells by directly targeting to the 3'UTR of ECM1 gene in a dual-luciferase reporter assay. These results highlighted the antagonistic roles of ECM1 and miR-92a in GC development, which may serve as a new target for gastric cancer.
ISSN:1873-6351
DOI:10.1016/j.fct.2019.110779