In vitro and in vivo study of the toxicity of fullerenols С 60 , С 70 and С 120 О obtained by an original two step method

• Published in: Materials science & engineering. C, Materials for biological applications Materials for biological applications, 2019-11, Vol.104, p.109945
• Main Authors: Bolshakova, Olga, Borisenkova, Alina, Suyasova, Marina, Sedov, Victor, Slobodina, Aleksandra, Timoshenko, Svetlana, Varfolomeeva, Elena, Golomidov, Ilia, Lebedev, Vasily, Aksenov, Victor, Sarantseva, Svetlana
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - drug effects; Cell Cycle - drug effects; Cell Line; Cell Line, Tumor; Cricetulus; Drosophila melanogaster - drug effects; Fullerenes - chemistry; Fullerenes - toxicity; HeLa Cells; Humans; Hydroxylation - drug effects
• ISSN: 1873-0191
• DOI: 10.1016/j.msec.2019.109945

The toxicity of C (OH) , C (OH) , and C O(OH) fullerenols, prepared by a new original method, has been studied. This method allowed us to obtain high-purity fullerenols and eliminate the risks of synthesis of preparations containing insoluble fractions contaminated with impurities such as fullerenes not completely reacted by hydroxylation. All fullerenols were detected inside cultured cells. The MTT assay as well as the analysis of apoptosis and cell cycle showed that С (ОН) and С (OH) are non-toxic for cultured V79 и HeLa cells at concentrations exceeding physiological levels by an order of magnitude. С O(OH) caused low toxicity. Studies in Drosophila melanogaster showed that any preparations used did not result in a decreased lifespan or in behavior abnormalities in flies.