Fast MAS 1 H- 13 C correlation NMR for structural investigations of plant cell walls

• Published in: Journal of biomolecular NMR 2019-12, Vol.73 (12), p.661
• Main Authors: Phyo, Pyae, Hong, Mei
• Format: Article
• Language: English
• Subjects: Arabidopsis - chemistry; Carbon-13 Magnetic Resonance Spectroscopy; Cell Wall - chemistry; Nuclear Magnetic Resonance, Biomolecular - methods; Plant Cells - ultrastructure; Polysaccharides - chemistry; Proton Magnetic Resonance Spectroscopy
• ISSN: 1573-5001
• DOI: 10.1007/s10858-019-00277-x

Plant cell walls consist of a mixture of polysaccharides that render the cell wall a strong and dynamic material. Understanding the molecular structure and dynamics of wall polysaccharides is important for understanding and improving the properties of this energy-rich biomaterial. So far, solid-state NMR studies of cell wall structure and dynamics have solely relied on C chemical shifts measured from 2D and 3D correlation experiments. To increase the spectral resolution, sensitivity and upper limit of measurable distances, it is of interest to explore H chemical shifts and H-detected NMR experiments for analyzing cell walls. Here we demonstrate 2D and 3D H- C correlation experiments at both moderate and fast MAS frequencies of 10-50 kHz to resolve and assign H chemical shifts of matrix polysaccharides in Arabidopsis primary cell walls. Both C-detected and H-detected experiments are implemented and are shown to provide useful and complementary information. Using the assigned H chemical shifts, we measured long-range correlations between matrix polysaccharides and cellulose using H- H instead of C- C spin diffusion, and the 2D experiments can be conducted with either C or H detection.