Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection

• Published in: Biofouling (Chur, Switzerland) Switzerland), 2019-10, Vol.35 (9), p.997-1006
• Main Authors: Rossoni, Rodnei Dennis, de Barros, Patrícia Pimentel, Lopes, Lucas Alexandre das Chagas, Ribeiro, Felipe Camargo, Nakatsuka, Toshiyuki, Kasaba, Hideto, Junqueira, Juliana Campos
• Format: Article
• Language: English
• Subjects: Acrylic Resins - pharmacology; Animals; Antifungal activity; Antifungal Agents - pharmacology; Antifungal Agents - toxicity; Bioactive compounds; Biocompatibility; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Candida; Candida - drug effects; Candida - growth & development; Candida albicans; Candida krusei; Candida spp; Candidiasis; Candidiasis, Oral - prevention & control; Dental materials; Fungicides; Galleria mellonella; Glass; Glass Ionomer Cements - pharmacology; Glass Ionomer Cements - toxicity; Hemocytes; In vivo methods and tests; Ionomers; Larva - drug effects; Larvae; Microbial Sensitivity Tests; Microbial Viability - drug effects; Minimum inhibitory concentration; Moths - drug effects; Moths - microbiology; Pathogens; S-PRG filler; Silicon Dioxide - pharmacology; Toxicity
• ISSN: 0892-7014; 1029-2454
• DOI: 10.1080/08927014.2019.1686485

Surface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against Candida, the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis. For the in vivo study, Galleria mellonella was used as a model to evaluate the effects of S-PRG on toxicity, hemocyte counts and candidiasis. The MIC of S-PRG ranged from 5 to 40% (v/v). S-PRG eluate exhibited anti-biofilm activity for all the Candida species tested. Furthermore, injection of S-PRG eluate into G. mellonella was not toxic to the larvae and protected G. mellonella against experimental candidiasis. In addition, S-PRG eluate inhibited biofilm formation by C. albicans, C. glabrata, C. krusei, and C. tropicalis and exerted protective effects on G. mellonella against experimental candidiasis in vivo.