CE determination of the thermodynamic pK a values and limiting ionic mobilities of 14 low molecular mass UV absorbing ampholytes for accurate characterization of the pH gradient in carrier ampholytes-based IEF and its numeric simulation

Fourteen low molecular mass UV absorbing ampholytes containing 1 or 2 weakly acidic and 1 or 2 weakly basic functional groups that best satisfy Rilbe's requirement for being good carrier ampholytes (ΔpK = pKa - pKa

Saved in:
Bibliographic Details
Published in:Electrophoresis 2020-04, Vol.41 (7-8), p.514
Main Authors: Ansorge, Martin, Gaš, Bohuslav, Boublík, Milan, Malý, Michal, Šteflová, Jana, Hruška, Vlastimil, Vigh, Gyula
Format: Article
Language:English
Subjects:
Ampholyte Mixtures - chemistry
Buffers
Computer Simulation
Electrophoresis, Capillary - methods
Hydrogen-Ion Concentration
Isoelectric Focusing - methods
Osmolar Concentration
Thermodynamics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fourteen low molecular mass UV absorbing ampholytes containing 1 or 2 weakly acidic and 1 or 2 weakly basic functional groups that best satisfy Rilbe's requirement for being good carrier ampholytes (ΔpK = pKa - pKa
ISSN:1522-2683
DOI:10.1002/elps.201900381