Effects of prostaglandin F 2α (PGF 2α ) on cell-death pathways in the bovine corpus luteum (CL)

• Published in: BMC veterinary research 2019-11, Vol.15 (1), p.416
• Main Authors: Jonczyk, Agnieszka Walentyna, Piotrowska-Tomala, Katarzyna Karolina, Skarzynski, Dariusz Jan
• Format: Article
• Language: English
• Subjects: Animals; Caspase 3 - genetics; Caspase 3 - metabolism; Caspase 8 - genetics; Caspase 8 - metabolism; Cattle; Cell Death - drug effects; Corpus Luteum - cytology; Corpus Luteum - drug effects; Corpus Luteum - physiology; Dinoprost - administration & dosage; Dinoprost - pharmacology; Drug Administration Schedule; Female; Gene Expression Regulation - drug effects; Oxytocics - pharmacology; Progesterone - blood; Progesterone - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism
• ISSN: 1746-6148
• DOI: 10.1186/s12917-019-2167-3

Prostaglandin F (PGF ) may differentially affect viability of luteal cells by inducing either proliferation or cell death (via apoptosis or necroptosis). The diverse effects of PGF may depend on its local vs. systemic actions. In our study, we determined changes in expression of genes related to: (i) apoptosis: caspase (CASP) 3, CASP8, BCL2 associated X (BAX), B-cell lymphoma 2 (BCL2) and (ii) necroptosis: receptor-interacting protein kinase (RIPK) 1, RIPK3, cylindromatosis (CYLD), and mixed lineage kinase domain-like (MLKL) in the early and mid-stage corpus luteum (CL) that accompany local (intra-CL) vs. systemic (i.m.) analogue of PGF (aPGF ) actions. Cows at day 4 (n = 24) or day 10 (n = 24) of the estrous cycle were treated by injections as follows: (1) systemic saline, (2) systemic aPGF (25 mg; Dinoprost), (3) local saline, (4) local aPGF (2.5 mg; Dinoprost). After 4 h, CLs were collected by ovariectomy. Expression levels of mRNA and protein were investigated by RT-q PCR, Western blotting and immunohistochemistry, respectively. We found that local and systemic administration of aPGF in the early-stage CL resulted in decreased expression of CASP3 (P