A study on the utility of immobilized cells of indigenous bacteria for biodegradation of reactive azo dyes

Azo dyes are recalcitrant compounds used as a colorant in various industries. The pollution caused by their extensive usage has adversely affected the environment for years. The existing physicochemical methods for dye pollution remediation are rather inefficient and hence there is a dearth of low-c...

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Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2020-04, Vol.50 (4), p.317-329
Main Authors: Pandey, Koushik, Saha, Purbasha, Rao, K. V. Bhaskara
Format: Article
Language:English
Subjects:
Alginic acid
Azo dyes
Bacillus
Bacteria
biodecolorization
Biodegradation
biotoxicity assay
Calcium alginate
Color removal
Decoloring
Decolorization
Dyes
Fourier transforms
High performance liquid chromatography
Immobilization
Immobilized cells
Infrared spectroscopy
Liquid chromatography
Lysinibacillus
Phytotoxicity
phytotoxicity assay
Pollution
Shaking
Sludge
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Summary:Azo dyes are recalcitrant compounds used as a colorant in various industries. The pollution caused by their extensive usage has adversely affected the environment for years. The existing physicochemical methods for dye pollution remediation are rather inefficient and hence there is a dearth of low-cost, potential systems capable of dye degradation. The current research studies the biodegradation potential of immobilized bacterial cells against azo dyes Reactive Orange 16 (RO-16) and Reactive Blue 250 (RB-250). Two indigenous dye degrading bacteria Bacillus sp. VITAKB20 and Lysinibacillus sp. KPB6 was isolated from textile sludge sample. Free cells of Bacillus. sp. VITAKB20 degraded 92.38% of RO-16 and that of Lysinibacillus sp. KPB6 degraded 95.36% of RB-250 within 72 h under static conditions. Upon immobilization with calcium alginate, dye degradation occurred rapidly. Bacillus. sp. VITAKB20 degraded 97.5% of RO-16 and Lysinibacillus sp. KPB6 degraded 98.2% of RB-250 within 48 h under shaking conditions. Further, the nature of dye decolorization was biodegradation as evident by high-performance liquid chromatography (HPLC), and Fourier-transform infrared spectroscopy (FTIR) results. Phytotoxicity and biotoxicity assays revealed that the degraded dye products were less toxic in nature than the pure dyes. Thus, immobilization proved to be a highly likely alternative treatment for dye removal.
ISSN:1082-6068
1532-2297
DOI:10.1080/10826068.2019.1692219