In vivo third-harmonic generation microscopy study on vitiligo patients

Melanin is known to provide strong third-harmonic generation (THG) contrast in human skin. With a high concentration in basal cell cytoplasm, THG contrast provided by melanin overshadows other THG sources in human skin studies. For better understanding of the THG signals in keratinocytes without the...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical optics 2020-01, Vol.25 (1), p.014504-014504
Main Authors: Liao, Yi-Hua, Su, Yu-Hsiang, Shih, Yuan-Ta, Chen, Wen-Shiang, Jee, Shiou-Hwa, Sun, Chi-Kuang
Format: Article
Language:English
Subjects:
Basal cells
Biopsy
Collagen
Cytoplasm
Hair
Harmonic generations
In vivo methods and tests
Keratinocytes
Lasers
Melanin
Microscopy
Narrowband
Skin
Skin diseases
Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
Vitiligo
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Melanin is known to provide strong third-harmonic generation (THG) contrast in human skin. With a high concentration in basal cell cytoplasm, THG contrast provided by melanin overshadows other THG sources in human skin studies. For better understanding of the THG signals in keratinocytes without the influence of melanin, an in vivo THG microscopy (THGM) study was first conducted on vitiliginous skin. As a result, the THG-brightness ratio between the melanin-lacking cytoplasm of basal cells and collagen fibers is about 1.106 at the dermal–epidermal junctions of vitiliginous skin, indicating high sensitivity of THGM for the presence of melanin. We further applied the in vivo THGM to assist evaluating the therapeutic outcome from the histopathological point of view for those showed no improvement under narrowband ultraviolet B therapy based on the seven-point Physician Global Assessment score. Our clinical study indicates the high potential of THGM to assist the histopathological assessment of the therapeutic efficacy of vitiligo treatments.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.25.1.014504