Overexpression of Regucalcin Suppresses the Growth of Human Osteosarcoma Cells in Vitro: Repressive Effect of Extracellular Regucalcin

• Published in: Cancer investigation 2020-01, Vol.38 (1), p.37-51
• Main Authors: Yamaguchi, Masayoshi, Murata, Tomiyasu
• Format: Article
• Language: English
• Subjects: Animals; Bone Neoplasms - genetics; Bone Neoplasms - pathology; Bone Neoplasms - therapy; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - isolation & purification; Calcium-Binding Proteins - metabolism; Carboxylic Ester Hydrolases - genetics; Carboxylic Ester Hydrolases - isolation & purification; Carboxylic Ester Hydrolases - metabolism; carcinogenesis; cell death; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; Genetic Therapy - methods; Humans; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Liver; osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - pathology; Osteosarcoma - therapy; Prognosis; Rats; Regucalcin; Transfection
• ISSN: 0735-7907; 1532-4192
• DOI: 10.1080/07357907.2019.1708924

Regucalcin plays a pivotal role as a suppressor of human carcinogenesis, and downregulation of regucalcin expression may contribute to the promotion of human osteosarcoma. Overexpression of regucalcin suppressed the proliferation of Saos-2 human osteosarcoma cells in vitro and decreased the protein levels of multiple signaling components, transcription factors, and tumor suppressors. Interestingly, extracellular regucalcin repressed colony formation and proliferation of Saos-2 cells, and reduced the protein levels of multiple signaling components, cell cycle inhibitor, and various transcription factors. Thus, regucalcin suppressed the growth of human osteosarcoma cells, providing a novel strategy with the gene therapy for treatment of osteosarcoma.