Toxicological evaluation of the herbicide Palace® in Drosophila melanogaster

• Published in: Journal of Toxicology and Environmental Health, Part A Part A, 2019-11, Vol.82 (22), p.1172-1185
• Main Authors: Leão, Mayara B., Gonçalves, Débora F., Miranda, Gabriela M., da Paixão, Giovanna M. X., Dalla Corte, Cristiane L.
• Format: Article
• Language: English
• Subjects: 2,4-D; 24-D; Acetylcholinesterase; behavioral toxicology; Bioenergetics; developmental toxicity; Dichlorophenoxyacetic acid; Dilution; Drosophila melanogaster; Ecosystem assessment; Environmental studies; Exposure; Females; Flies; Herbicides; Insects; Larvae; Males; Mitochondria; mitochondrial dysfunction; Oxidative phosphorylation; Oxygen consumption; Palaces; Pesticides; Phosphorylation; Picloram; Pollutants; Pollution studies; Protein thiols; Proteins; Reactive oxygen species; Respiration; Side effects; Survival; Thiols; Toxicity testing
• ISSN: 1528-7394; 1087-2620; 2381-3504
• DOI: 10.1080/15287394.2019.1709109

Drosophila melanogaster is a suitable model for toxicological studies of environmental pollutants including pesticides, which are known to produce adverse effects on the ecosystem. The aim of the present study was to investigate the adverse influence of the pesticide Palace®, a mixture of 2,4-dichlorophenoxyacetic acid (2,4-D) and picloram, using D. melanogaster as a model organism. D. melanogaster larvae were exposed to 0.011%, 0.022%, 0.112%, 0.224%, and 1.12% of Palace® and development examined. Adult flies were treated with 0.224%, 1.12%, 2.24%, 11.2%, and 22.4% of Palace® and the following analyzed survival, locomotor behavior, acetylcholinesterase (AchE) activity, reactive oxygen species (ROS) production, total and non-protein thiol levels, and mitochondrial function. Data demonstrated that exposure of flies during larval stage to Palace® significantly affected development of larvae to the adult stage. In adults, treatment with Palace® resulted in dose-dependent progressive adverse effects on survival and behavior with males more sensitive than females. In both males and females, ROS production and AchE activity were not markedly affected by Palace®. However, total thiol levels increased in female heads treated with highest dilution of Palace®, while decreased levels of non-protein thiols were detected in heads of male flies following Palace® exposure. In females and males flies exposed to Palace® reduced mitochondrial oxygen consumption related to oxidative phosphorylation (OXPHOS) state, mitochondrial capacity of excess (E-P) and respiratory control ratio (RCR) was noted, indicating that the pesticide mixture altered mitochondrial complexes functionality with consequences on bioenergetics. In summary, Palace® exposure produced adverse effects on D. melanogaster affecting survival, development, behavior and mitochondrial function, which may exert ecotoxicological consequences which poses risks to different organisms in the ecosystem.