Protective Immunity in Mice Immunized With P. vivax MSP1 19 -Based Formulations and Challenged With P. berghei Expressing Pv MSP1 19

The lack of continuous cultures has been an obstacle delaying pre-clinical testing of vaccine formulations based on known antigens. In this study, we generated a model to test available formulations based on the MSP1 antigen. The strains ANKA and NK65 were modified to express MSP1 instead of the end...

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Bibliographic Details
Published in:Frontiers in immunology 2020, Vol.11, p.28
Main Authors: Dobrescu, Irina, de Camargo, Tarsila Mendes, Gimenez, Alba Marina, Murillo, Oscar, Amorim, Kelly Nazaré da Silva, Marinho, Claudio Romero Farias, Soares, Irene Silva, Boscardin, Silvia Beatriz, Bargieri, Daniel Youssef
Format: Article
Language:English
Subjects:
Animals
Antibodies, Protozoan - immunology
Antigens, Protozoan - immunology
Female
Immunogenicity, Vaccine
Malaria Vaccines - immunology
Malaria, Vivax - immunology
Malaria, Vivax - parasitology
Merozoite Surface Protein 1 - immunology
Merozoite Surface Protein 1 - metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Parasitemia - immunology
Plasmids - genetics
Plasmodium berghei - genetics
Plasmodium berghei - metabolism
Plasmodium vivax - immunology
Protozoan Proteins - immunology
Transfection
Treatment Outcome
Vaccination
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Summary:The lack of continuous cultures has been an obstacle delaying pre-clinical testing of vaccine formulations based on known antigens. In this study, we generated a model to test available formulations based on the MSP1 antigen. The strains ANKA and NK65 were modified to express MSP1 instead of the endogenous MSP1 . The hybrid parasites were used to challenge C57BL/6 or BALB/c mice immunized with MSP1 -based vaccine formulations. The MSP1 was correctly expressed in the hybrid mutant lines as confirmed by immunofluorescence using anti- MSP1 monoclonal antibodies and by Western blot. Replacement of the MSP1 by the MSP1 had no impact on asexual growth . High titers of specific antibodies to MSP1 were not sufficient to control initial parasitemia in the immunized mice, but late parasitemia control and a balanced inflammatory process protected these mice from dying, suggesting that an established immune response to MSP1 in this model can help immunity mounted later during infection.
ISSN:1664-3224
DOI:10.3389/fimmu.2020.00028