Improving resolving ability of expansion microscopy by varying crosslinker concentration

Here, we systematically investigated the performance of expansion microscopy (ExM) with different crosslinker concentrations. We modified ExM with 0.06% N , N ′-methylenebisacrylamide (MBAA) (termed 0.06%-MBAA ExM), increased the expansion factor to 5.7 and achieved a lateral resolution of ∼50 nm wi...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2020-04, Vol.56 (3), p.4176-4179
Main Authors: Hu, Yanlei, Chu, Ximing, Chen, Ting-ting, Pan, Qingshan, Liu, Chang, Yi, Jintao, Chu, Xia
Format: Article
Language:English
Subjects:
Crosslinking
Methylene bisacrylamide
Microscopy
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Summary:Here, we systematically investigated the performance of expansion microscopy (ExM) with different crosslinker concentrations. We modified ExM with 0.06% N , N ′-methylenebisacrylamide (MBAA) (termed 0.06%-MBAA ExM), increased the expansion factor to 5.7 and achieved a lateral resolution of ∼50 nm with a common confocal microscope. Here, we systematically investigated the performance of expansion microscopy (ExM) with different crosslinker concentrations.
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc00052c