Improvement of a miRNA inhibitor by intracellular selection

Sequences surrounding the miRNA binding domain of the miRNA inhibitor LidNA were selected intracellularly. The library was transfected into cells, and then, inhibitors that were associated with argonaute 2 were selected. The potent inhibitors were slowly degraded intracellularly, while the lower-act...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2020-07, Vol.84 (7), p.1451-1454
Main Authors: Tachibana, Akira, Yamamoto, Aiko
Format: Article
Language:English
Subjects:
Ago2
Argonaute Proteins - metabolism
Base Sequence
DNA Probes - chemistry
DNA Probes - genetics
DNA Probes - pharmacology
DNA, Single-Stranded - chemistry
DNA, Single-Stranded - metabolism
HEK293 Cells
Humans
LidNA
MicroRNAs - antagonists & inhibitors
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
miRNA inhibitor
Oligonucleotides - pharmacology
RNA-Binding Motifs
Transfection
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Summary:Sequences surrounding the miRNA binding domain of the miRNA inhibitor LidNA were selected intracellularly. The library was transfected into cells, and then, inhibitors that were associated with argonaute 2 were selected. The potent inhibitors were slowly degraded intracellularly, while the lower-activity inhibitors were rapidly degraded. A combination of the selected sequences surrounding the miRNA binding domain enhanced miRNA inhibitory activity. LidNA: DNA that puts a lid on miRNA function; LNA: locked nucleic acid; Ago2: argonaute 2; LNA: locked nucleic acid
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2020.1743167