Analysis of illegal colourants (citrus red II, diethyl yellow, dimethyl yellow, metanil yellow and rhodamine B) in foods by LC-UV and LC-MS/MS

Synthetic colourants are highly regulated due to their correlation with a variety of health hazards. Regulatory services must be able to detect the substances in a cost-effective, efficient, and sensitive manner. LC-UV and LC-MS/MS methods have been developed to simultaneously detect five illegal co...

Full description

Saved in:
Bibliographic Details
Published in:Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2020-06, Vol.37 (6), p.895-904
Main Authors: Lim, Ho Soo, Choi, EunA, Lee, Jin-Hye, Lee, Gunyoung, Kim, MeeKyung
Format: Article
Language:English
Subjects:
Azo Compounds - analysis
Beverages
Chromatography, Liquid
Coloring agents
Food
Food Analysis
Food Coloring Agents - analysis
Food Contamination - analysis
Health hazards
HPLC
Illegal food colourant
Inspection
LC-MS/MS
Linearity
method validation
Quantitation
Rhodamine
Rhodamines - analysis
Spectrophotometry, Ultraviolet
Tandem Mass Spectrometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Synthetic colourants are highly regulated due to their correlation with a variety of health hazards. Regulatory services must be able to detect the substances in a cost-effective, efficient, and sensitive manner. LC-UV and LC-MS/MS methods have been developed to simultaneously detect five illegal colourants in foods, such as: citrus red II, diethyl yellow, dimethyl yellow, metanil yellow, and rhodamine B. This method showed good linearity (R 2  > 0.99) and low limits of detection (0.09-0.19 mg kg -1 ) and quantitation (0.26-0.58 mg kg -1 ). The recoveries at three standard concentration levels ranged between 80.9% and 120%, with relative standard deviations below 12%. The expanded uncertainties determined for the five colourants in three food matrices were 8.2-19.4%. This method was applied to monitor five illegal colourants in imported and domestic beverages, candies, and sauces. None of the five colourants were found in any of the 510 samples. The method is suitable for quantitative analysis of five illegal colourants simultaneously in various foods and can be applied to improve current surveillance and inspection services.
ISSN:1944-0049
1944-0057
DOI:10.1080/19440049.2020.1746840