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Disodium cromoglycate treatment reduces T H 2 immune response and immunohistopathological features in a murine model of Eosinophilic Esophagitis

Eosinophilic esophagitis (EoE) is an emergent chronic disease of the esophagus. The immunopathological process in EoE is characterized by Th2 immune response and prominent eosinophilic influx, in response to common food allergens. The classical treatment consists of allergen elimination diet and sys...

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Bibliographic Details
Published in:International immunopharmacology 2020-06, Vol.83, p.106422
Main Authors: Silva, Flávia Márcia de Castro E, de Oliveira, Erick Esteves, Ambrósio, Marcilene Gomes Evangelista, Ayupe, Marina Caçador, de Souza, Viviane Passos, Menegati, Laura Machado, Reis, Daniele Ribeiro de Lima, Machado, Marco Antonio, Macedo, Gilson Costa, Ferreira, Ana Paula
Format: Article
Language:English
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Summary:Eosinophilic esophagitis (EoE) is an emergent chronic disease of the esophagus. The immunopathological process in EoE is characterized by Th2 immune response and prominent eosinophilic influx, in response to common food allergens. The classical treatment consists of allergen elimination diet and systemic/topical corticosteroid therapy. Nevertheless, patients do not always comply to treatment, and the prolonged corticosteroid therapy can cause side effects, therefore, there is an immediate need for new therapeutic approach for EoE. Disodium cromoglicate (DSCG) is a substance broadly used in allergic asthma treatment, and a well-known mast cell activation stabilizer. However, its effect in EoE have not been evaluated yet. This study aimed to assess the effects of DSCG treatment in an EoE experimental model. Male Balb/C mice were subcutaneously sensitized for five days with OVA, and subsequently orally OVA-challenged, DSCG administration was performed between the OVA-challenges. DSCG treatment not only reduced eosinophilic and mast cell influx, as well as reduced fibrosis. In addition, tslp, GATA IL-5, FoxP and IL-10 mRNA expression were reduced in esophageal mucosa, associated with lower Th2 (CD3 CD4 GATA3 IL4 ) and B cells (CD19 CD40 ) number in peripheral lymphoid organs. In conclusion, the data demonstrate DSCG treatment was effective in reducing mast cell activation and Th2 immune response, important immunopathological EoE features. Therefore, the use of DSCG as an EoE treatment can be considered a promising therapeutic approach to treat this disease.
ISSN:1878-1705