Electrochemical sensing of urinary progesterone with molecularly imprinted poly(aniline--metanilic acid)s

• Published in: Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2016-06, Vol.4 (21), p.3782-3787
• Main Authors: Lee, Mei-Hwa, O'Hare, Danny, Guo, Han-Zhang, Yang, Chien-Hsin, Lin, Hung-Yin
• Format: Article
• Language: English
• Subjects: Biology; Chips; Creatinine; Detection; Electrodes; Sex hormones; Ureas; Urine
• ISSN: 2050-750X; 2050-7518
• DOI: 10.1039/c6tb00760k

In this work, progesterone is imprinted into poly(aniline- co -metanilic acid) on the working electrode of an electrochemical sensing chip. This sensing chip was used directly to optimize the composition of the imprinting polymer. Poly(aniline- co -metanilic acid) deposited from a 1 : 3 molar ratio of aniline (ANI) :  m -aminobenzenesulfonic acid (MSAN) had an imprinting effectiveness which led to a four-fold greater electrochemical response than pure polyaniline. The electrochemical sensing of progesterone had a limit of detection (LOD) less than 1.0 pg mL −1 , and the direct electrochemical response was very weak even at high interference concentrations. Results from potential interferents (urea, testosterone, creatinine and 17-β estradiol) are reported. The progesterone levels that were measured in a random urine analysis were compared with those obtained using a commercial ARCHITECT system, and the accuracy of the progesterone concentration was 89.0 ± 5.3% at a concentration of 0.64-5.27 ng mL −1 . The sensing of urinary progesterone with molecularly imprinted poly(aniline- co -metanilic acid) coated electrodes.