In vitro activity of the antimicrobial peptide AP7121 against the human methicillin-resistant biofilm producers Staphylococcus aureus and Staphylococcus epidermidis

In vitro activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis biofilm producers from blood cultures of patients with prosthetic hip infections was evaluated. The Minimum Inhibitory Concentration (MIC) for AP7121 was determined and the bactericidal activi...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2020-03, Vol.36 (3), p.266-275
Main Authors: Delpech, Gastón, Ceci, Mónica, Lissarrague, Sabina, García Allende, Leonardo, Baldaccini, Beatriz, Sparo, Mónica
Format: Article
Language:English
Subjects:
antimicrobial peptide AP7121
Antimicrobial peptides
Bactericidal activity
biofilm
Biofilms
Drug resistance
Electron microscopy
Hip
Methicillin
methicillin-resistant Staphylococcus aureus
Minimum inhibitory concentration
Peptides
Planktonic cells
Prevention
Prostheses
S. epidermidis
Scanning electron microscopy
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus infections
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Summary:In vitro activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis biofilm producers from blood cultures of patients with prosthetic hip infections was evaluated. The Minimum Inhibitory Concentration (MIC) for AP7121 was determined and the bactericidal activity of AP7121 (MICx1, MICx4) against planktonic cells was studied at 4, 8 and 24 h. The biofilms formed were incubated with AP7121 (MICx1, MICx4) for 1 and 24 h. The anti-adhesion effect of an AP7121-treated inert surface over the highest MIC isolate was studied with scanning electron microscopy (SEM). The bactericidal activity of AP7121 against all the planktonic staphylococcal cells was observed at 4 h at both peptide concentrations. Dose-dependent anti-biofilm activity was detected. AP7121 (MICx4) showed bactericidal activity at 24 h in all isolates. SEM confirmed prevention of biofilm formation. This research showed the in vitro anti-biofilm activity of AP7121 against MRSA and S. epidermidis and the prevention of biofilm formation by them on an abiotic surface.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2020.1756266