Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery

Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternativ...

Full description

Saved in:
Bibliographic Details
Published in:Journal of extracellular vesicles 2020-01, Vol.9 (1), p.1747206-n/a
Main Authors: Saari, Heikki, Turunen, Tiia, Lõhmus, Andres, Turunen, Mikko, Jalasvuori, Matti, Butcher, Sarah J., Ylä-Herttuala, Seppo, Viitala, Tapani, Cerullo, Vincenzo, Siljander, Pia R. M., Yliperttula, Marjo
Format: Article
Language:English
Subjects:
adenovirus
Adenoviruses
Apoptosis
Cancer
Cancer therapies
cancer therapy
Cytotoxicity
Detergents
DNA delivery
DNA viruses
Extracellular vesicles
Gene therapy
Immune system
Infections
Oncolysis
Proteins
Structure-function relationships
Viral infections
Virions
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy.
ISSN:2001-3078
2001-3078
DOI:10.1080/20013078.2020.1747206