Toxicity evaluation and microbiota response of the lined sole Achirus lineatus (Chordata: Achiridae) exposed to the light petroleum water-accommodated fraction (WAF)

• Published in: Journal of Toxicology and Environmental Health, Part A Part A, 2020-04, Vol.83 (8), p.313-329
• Main Authors: Améndola-Pimenta, Mónica, Cerqueda-García, Daniel, Zamora-Briseño, Jesús A., Couoh-Puga, Danilú, Montero-Muñoz, Jorge, Árcega-Cabrera, Flor, Ceja-Moreno, Víctor, Pérez-Vega, Juan A., García-Maldonado, José Q., del Río-García, Marcela, Zapata-Pérez, Omar, Rodríguez-Canul, Rossanna
• Format: Article
• Language: English
• Subjects: Achirus lineatus; Acute toxicity; Adults; Animals; Aquatic organisms; Atrophy; Bile; Bile - chemistry; Contaminants; Crude oil; Degeneration; Disintegration; Dysbacteriosis; Exposure; Flatfishes - microbiology; Gills; Hyperplasia; Indigenous species; Intestinal microflora; lined sole; Metabolites; Microbiomes; Microbiota; Microbiota - drug effects; Nodules; Organisms; Petroleum; Petroleum - analysis; Petroleum - toxicity; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - metabolism; Polycyclic Aromatic Hydrocarbons - toxicity; Spleen; Toxicity; Toxicity Tests; Water Pollutants, Chemical - toxicity; Water pollution; water-accommodated fraction
• ISSN: 1528-7394; 1087-2620; 2381-3504
• DOI: 10.1080/15287394.2020.1758861

Exposure to contaminants might directly affect organisms and alter their associated microbiota. The objective of the present study was to determine the impact of the petroleum-water-accommodated fraction (WAF) from a light crude oil (API gravity 35) on a benthic fish species native from the Gulf of Mexico (GoM). Ten adults of Achirus lineatus (Linnaeus, 1758) were exposed to a sublethal WAF/water solution of 50% v/v for 48 hr. Multiple endpoints were measured including tissue damage, presence of polycyclic aromatic hydrocarbons (PAHs) metabolites in bile and gut microbiota analyses. Atrophy and fatty degeneration were observed in livers. Nodules and inflammation were detected in spleen, and structural disintegration and atrophy in the kidney. In gills hyperplasia, aneurysm, and gills lamellar fusion were observed. PAHs metabolites concentrations in bile were significantly higher in exposed organisms. Gut microbiome taxonomic analysis showed significant shifts in bacterial structure and composition following WAF exposure. Data indicate that exposure to WAF produced toxic effects in adults of A. lineatus, as evidenced by histological alterations and dysbiosis, which might represent an impairment to long-term subsistence of exposed aquatic organisms.