Fluorescence imaging-guided multifunctional liposomes for tumor-specific phototherapy for laryngeal carcinoma

• Published in: Biomaterials science 2020-06, Vol.8 (12), p.3443-3453
• Main Authors: Wu, Di, Zhao, Zheng, Wang, Nan, Zhang, Xinrui, Yan, Honghong, Chen, Xiaoqi, Fan, Yunyun, Liu, Weiwei, Liu, Xuekui
• Format: Article
• Language: English
• Subjects: Ablation; Animals; Biocompatibility; Blood circulation; Cell Line, Tumor; Coloring Agents - administration & dosage; Coloring Agents - chemistry; Coloring Agents - pharmacokinetics; Fluorescence; Humans; Hyperthermia; Imaging; Indocyanine Green - administration & dosage; Indocyanine Green - chemistry; Indocyanine Green - pharmacokinetics; Laryngeal Neoplasms - metabolism; Laryngeal Neoplasms - pathology; Laryngeal Neoplasms - therapy; Lasers; Light therapy; Lipids; Liposomes; Male; Mice, SCID; Nanoparticles; Oligopeptides - administration & dosage; Oligopeptides - chemistry; Oligopeptides - pharmacokinetics; Optical Imaging; Phosphatidylethanolamines - administration & dosage; Phosphatidylethanolamines - chemistry; Phosphatidylethanolamines - pharmacokinetics; Photodynamic therapy; Phototherapy; Polyethylene Glycols - administration & dosage; Polyethylene Glycols - chemistry; Polyethylene Glycols - pharmacokinetics; Reactive Oxygen Species - metabolism; Toxicity; Tumors
• ISSN: 2047-4830; 2047-4849
• DOI: 10.1039/d0bm00249f

Reliable diagnosis and efficient targeted therapy are important and may lead to the effective treatment of laryngeal carcinoma. Multifunctional nano-theranostic agents demonstrate great potential in tumor theranostic applications. Thus, herein, we report novel targeting multifunctional theranostic nanoparticles, internalized RGD (iRGD)-modified indocyanine green (ICG) encapsulated liposomes (iLIP ICG ), for imaging-guided photothermal therapy (PTT) and photodynamic therapy (PDT) for the treatment of laryngeal carcinoma. The iRGD-PEG-DSPE lipid endowed iLIP ICG with high affinity for tumor vascular targeting, tumor-penetration and tumor cell targeting. The in vivo results showed that iLIP ICG exhibited excellent blood circulation and tumor accumulation. iLIP ICG could be spatially and temporally controlled, simultaneously producing hyperthermia and reactive oxygen species as well as a fluorescence-guided effect through ICG to ablate laryngeal carcinoma cells under irradiation from an 808 nm laser. iLIP ICG generated synergistic photodynamic-photothermal cytotoxicity against Hep-2 cells, resulting in the efficient ablation of laryngeal carcinoma. Thus, the iLIP ICG system provides a promising strategy to improve the precision imaging and effective phototherapy for the treatment of laryngeal carcinoma. The tumor-targeting iLIP ICG provides a promising strategy to improve the precision imaging and effective phototherapy for the treatment of laryngeal carcinoma.