Uniform straw-like cell architecture for three-dimensional cell-cell communication assay

We fabricated uniform straw-like cell architecture with central lumen using a suture thread within 1 h. The architecture consisting of cancer cells and mature adipocyte was used for cell-cell communication assay, although mature adipocyte could not form spontaneous multi-cellular spheroids. Using th...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2020-08, Vol.84 (8), p.1681-1684
Main Authors: Inubushi, Yusaku, Sakaguchi, Yoshiki, Tachibana, Akira
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - metabolism
Biomarkers - metabolism
CD36 Antigens - genetics
CD36 Antigens - metabolism
Cell Communication - genetics
Cell Culture Techniques
Cell Differentiation
cell-cell communication
Coculture Techniques
cylindrical cell architecture
Fatty Acid-Binding Proteins - genetics
Fatty Acid-Binding Proteins - metabolism
Forkhead Box Protein M1 - genetics
Forkhead Box Protein M1 - metabolism
Gene Expression
Genes, Reporter
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
HEK293 Cells
Humans
MCF-7 Cells
Signal Transduction - genetics
Spheroids
Spheroids, Cellular - cytology
Spheroids, Cellular - metabolism
straw-like cell architecture
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Summary:We fabricated uniform straw-like cell architecture with central lumen using a suture thread within 1 h. The architecture consisting of cancer cells and mature adipocyte was used for cell-cell communication assay, although mature adipocyte could not form spontaneous multi-cellular spheroids. Using the system, it is possible to investigate three-dimensional cell-cell communication as an alternative to animal experiments. EB: embryoid body; 2D: two-dimension; 3D: three-dimension; PBS: phosphate buffered saline
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2020.1764330