Basement membrane recovery process in rat soleus muscle after exercise-induced muscle injury

Purpose: Collagen IV is a component of the basement membrane (BM) that provides mechanical support for muscle fibers. In addition, transcription factor 4 (TCF4) is highly expressed in muscle connective tissue fibroblasts and regulates muscle regeneration. However, the expression of collagen IV and T...

Full description

Saved in:
Bibliographic Details
Published in:Connective tissue research 2021-09, Vol.62 (5), p.519-530
Main Authors: Kanazawa, Yuji, Nagano, Mamoru, Koinuma, Satoshi, Sujino, Mitsugu, Minami, Yoichi, Sugiyo, Shinichi, Takeda, Isao, Shigeyoshi, Yasufumi
Format: Article
Language:English
Subjects:
Animals
Basement Membrane
Collagen
fibroblast
Muscle Fibers, Skeletal
muscle injury
Muscle, Skeletal
Rats
Rats, Wistar
recovery
soleus muscle
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose: Collagen IV is a component of the basement membrane (BM) that provides mechanical support for muscle fibers. In addition, transcription factor 4 (TCF4) is highly expressed in muscle connective tissue fibroblasts and regulates muscle regeneration. However, the expression of collagen IV and TCF4 (+) cells in response to exercise-induced muscle injury is not well known. Here, we investigated the expression and localization of collagen IV and TCF4 (+) cells during the recovery process after muscle injury induced by different exercise loads. Materials and Methods: Muscle injury was observed in the soleus muscle of young Wistar rats after 12 or 18 sets-downhill running (DR) on a treadmill. After running, the rats were permitted to recover for a period of 0.5 days, 2 days, or 7 days. Results: Ectopic localization of collagen IV in injured muscle fibers was observed after DR, and the number increased at 0.5 days after 18 sets DR and at 2 days after 12 or 18 sets DR as compared to the number observed at baseline. BM disruption was observed after DR. TCF4 (+) cells appeared in the inside and around injured muscle fibers at 0.5 day of recovery. After 18 sets DR, TCF4 (+) cells were more abundant for a longer period than that observed after 12 sets DR. Conclusions: DR induces BM disruption accompanied by muscle fiber damage. It is possible that BM destruction may be accompanied by muscle damage and that TCF4 (+) cells contribute to muscle fiber and BM recovery.
ISSN:0300-8207
1607-8438
DOI:10.1080/03008207.2020.1791839