An off-on electrochemiluminescence detection for microRNAs based on TiO 2 nanotubes sensitized with gold nanoparticles as enhanced emitters

A sensitive electrochemiluminescence (ECL) assay for microRNAs (miRNAs) based on a semiconductor nanomaterial sensitized with noble-metal Au nanoparticles (NPs) is successfully developed. TiO nanotubes (NTs) were equipped with Au NPs to obtain an enhanced ECL emitter. Then, an ECL assay for miRNA-21...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2020-09, Vol.412 (23), p.5779
Main Authors: Dai, Panpan, Ke, Jiajun, Xie, Chenggen, Wei, Liyun, Zhang, Ying, He, Yong, Chen, Lijuan, Jin, Juncheng
Format: Article
Language:English
Subjects:
Biosensing Techniques
Dendrimers - chemistry
Electrochemical Techniques - methods
Gold - chemistry
Humans
Limit of Detection
Luminescent Measurements - methods
Metal Nanoparticles - chemistry
MicroRNAs - analysis
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanotubes - chemistry
Spectrophotometry, Ultraviolet
Titanium - chemistry
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Summary:A sensitive electrochemiluminescence (ECL) assay for microRNAs (miRNAs) based on a semiconductor nanomaterial sensitized with noble-metal Au nanoparticles (NPs) is successfully developed. TiO nanotubes (NTs) were equipped with Au NPs to obtain an enhanced ECL emitter. Then, an ECL assay for miRNA-21 was fabricated, which was based on the use of probe 2 DNA-functionalized Pt/PAMAM nanocomposites (NCs) assembled on the surface of Au/TiO NT conjugate via DNA hybridization between probe 1 DNA and capture DNA. The Pt/PAMAM NCs act as an ECL quencher of Au/TiO NTs via resonance energy transfer. After the binding of target miRNA-21 and the capture DNA, the Pt/PAMAM NCs were released and the ECL signal was recovered. An "off-on" ECL assay was achieved with a linear response from 0.01 to 10,000 pM. Finally, this method has been validated to be sensitive and specific for miRNAs in human serum samples. The ECL enhancement strategy opens a new way for fabricating various sensitive biosensors. Graphical abstract A sensitive "off-on" electrochemiluminescence analysis method was developed, which combined Au NP-enhanced ECL emission of TiO nanotubes and an efficient energy-transfer system between Au/TiO nanotubes and Pt/PAMAM nanocomposites.
ISSN:1618-2650
DOI:10.1007/s00216-020-02800-8