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Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. T...
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| Published in: | Virulence 2020-12, Vol.11 (1), p.964-967 |
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| Main Authors: | , , , , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c534t-6ea1e8216d83bd9c21473c5da4fc0d0207e40f2da22566ef9827cb1a04c7b1e03 |
| container_end_page | 967 |
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| container_title | Virulence |
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| creator | Mayer, Florian J. Ratzinger, Franz Schmidt, Ralf L.J. Greiner, Georg Landt, Olfert Am Ende, Alexander Corman, Victor M. Perkmann-Nagele, Nicole Watkins-Riedel, Thomas Petermann, Dagmar Abadir, Karoline Zweimüller-Mayer, Josef Strassl, Robert |
| description | Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84-97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time. |
| doi_str_mv | 10.1080/21505594.2020.1798041 |
| format | article |
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Ratzinger, Franz ; Schmidt, Ralf L.J. ; Greiner, Georg ; Landt, Olfert ; Am Ende, Alexander ; Corman, Victor M. ; Perkmann-Nagele, Nicole ; Watkins-Riedel, Thomas ; Petermann, Dagmar ; Abadir, Karoline ; Zweimüller-Mayer, Josef ; Strassl, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-6ea1e8216d83bd9c21473c5da4fc0d0207e40f2da22566ef9827cb1a04c7b1e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Betacoronavirus - genetics</topic><topic>Betacoronavirus - isolation & purification</topic><topic>Coronavirus</topic><topic>COVID-19</topic><topic>High-Throughput Nucleotide Sequencing - instrumentation</topic><topic>High-Throughput Nucleotide Sequencing - methods</topic><topic>High-Throughput Nucleotide Sequencing - standards</topic><topic>Humans</topic><topic>Real-Time Polymerase Chain Reaction - instrumentation</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Real-Time Polymerase Chain Reaction - standards</topic><topic>Reproducibility of Results</topic><topic>Research Paper</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>SARS-CoV-2</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mayer, Florian J.</creatorcontrib><creatorcontrib>Ratzinger, Franz</creatorcontrib><creatorcontrib>Schmidt, Ralf L.J.</creatorcontrib><creatorcontrib>Greiner, Georg</creatorcontrib><creatorcontrib>Landt, Olfert</creatorcontrib><creatorcontrib>Am Ende, Alexander</creatorcontrib><creatorcontrib>Corman, Victor M.</creatorcontrib><creatorcontrib>Perkmann-Nagele, Nicole</creatorcontrib><creatorcontrib>Watkins-Riedel, Thomas</creatorcontrib><creatorcontrib>Petermann, Dagmar</creatorcontrib><creatorcontrib>Abadir, Karoline</creatorcontrib><creatorcontrib>Zweimüller-Mayer, Josef</creatorcontrib><creatorcontrib>Strassl, Robert</creatorcontrib><collection>Access via Taylor & Francis (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Virulence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mayer, Florian J.</au><au>Ratzinger, Franz</au><au>Schmidt, Ralf L.J.</au><au>Greiner, Georg</au><au>Landt, Olfert</au><au>Am Ende, Alexander</au><au>Corman, Victor M.</au><au>Perkmann-Nagele, Nicole</au><au>Watkins-Riedel, Thomas</au><au>Petermann, Dagmar</au><au>Abadir, Karoline</au><au>Zweimüller-Mayer, Josef</au><au>Strassl, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed</atitle><jtitle>Virulence</jtitle><addtitle>Virulence</addtitle><date>2020-12</date><risdate>2020</risdate><volume>11</volume><issue>1</issue><spage>964</spage><epage>967</epage><pages>964-967</pages><issn>2150-5594</issn><eissn>2150-5608</eissn><abstract>Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. 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| ispartof | Virulence, 2020-12, Vol.11 (1), p.964-967 |
| issn | 2150-5594 2150-5608 |
| language | eng |
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| source | Access via Taylor & Francis (Open Access Collection); PubMed Central |
| subjects | Betacoronavirus - genetics Betacoronavirus - isolation & purification Coronavirus COVID-19 High-Throughput Nucleotide Sequencing - instrumentation High-Throughput Nucleotide Sequencing - methods High-Throughput Nucleotide Sequencing - standards Humans Real-Time Polymerase Chain Reaction - instrumentation Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Reproducibility of Results Research Paper RNA, Viral - genetics RNA, Viral - isolation & purification SARS-CoV-2 Sensitivity and Specificity Time Factors |
| title | Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed |
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