Reversible spatial and temporal control of lipid signaling

Herein, we introduce versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes. These molecules were prepared by tethering ortho -nitrobenzyl-based fluorescent cages with a signaling...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2020-09, Vol.56 (73), p.1646-1649
Main Authors: Gaur, Pankaj, Galkin, Maksym, Hauke, Sebastian, Redkin, Ruslan, Barnes, Carolyn, Shvadchak, Volodymyr V, Yushchenko, Dmytro A
Format: Article
Language:English
Subjects:
Azo compounds
Azo Compounds - chemistry
Azo Compounds - radiation effects
Calcium - metabolism
Calcium ions
Cell membranes
Deactivation
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent Dyes - radiation effects
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
Lipids
Microscopy, Confocal
Microscopy, Fluorescence
Receptors, G-Protein-Coupled - metabolism
Rhodamines - chemistry
Rhodamines - radiation effects
Signaling
Tethering
Ultraviolet Rays
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Summary:Herein, we introduce versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes. These molecules were prepared by tethering ortho -nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They permit two sequential photocontrolled reactions, which are uncaging of a lipid analogue and then its repeated activation and deactivation. We used these molecules to activate GPR40 receptor transiently expressed in HeLa cells and demonstrated downstream modulation of intracellular Ca 2+ levels. Fluorescent cages for selective delivery of photoswitchable lipids to specific cell membranes for high-precision studies of lipid signaling.
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc04146g