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Reversible spatial and temporal control of lipid signaling
Herein, we introduce versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes. These molecules were prepared by tethering ortho -nitrobenzyl-based fluorescent cages with a signaling...
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| Published in: | Chemical communications (Cambridge, England) England), 2020-09, Vol.56 (73), p.1646-1649 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c363t-c5ed46ede908fa80d07e5f74a708ccf7cb934a0f05e3379c5b6f3235f34253873 |
| container_end_page | 1649 |
| container_issue | 73 |
| container_start_page | 1646 |
| container_title | Chemical communications (Cambridge, England) |
| container_volume | 56 |
| creator | Gaur, Pankaj Galkin, Maksym Hauke, Sebastian Redkin, Ruslan Barnes, Carolyn Shvadchak, Volodymyr V Yushchenko, Dmytro A |
| description | Herein, we introduce versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes. These molecules were prepared by tethering
ortho
-nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They permit two sequential photocontrolled reactions, which are uncaging of a lipid analogue and then its repeated activation and deactivation. We used these molecules to activate GPR40 receptor transiently expressed in HeLa cells and demonstrated downstream modulation of intracellular Ca
2+
levels.
Fluorescent cages for selective delivery of photoswitchable lipids to specific cell membranes for high-precision studies of lipid signaling. |
| doi_str_mv | 10.1039/d0cc04146g |
| format | article |
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ortho
-nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They permit two sequential photocontrolled reactions, which are uncaging of a lipid analogue and then its repeated activation and deactivation. We used these molecules to activate GPR40 receptor transiently expressed in HeLa cells and demonstrated downstream modulation of intracellular Ca
2+
levels.
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ortho
-nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They permit two sequential photocontrolled reactions, which are uncaging of a lipid analogue and then its repeated activation and deactivation. We used these molecules to activate GPR40 receptor transiently expressed in HeLa cells and demonstrated downstream modulation of intracellular Ca
2+
levels.
Fluorescent cages for selective delivery of photoswitchable lipids to specific cell membranes for high-precision studies of lipid signaling.</description><subject>Azo compounds</subject><subject>Azo Compounds - chemistry</subject><subject>Azo Compounds - radiation effects</subject><subject>Calcium - metabolism</subject><subject>Calcium ions</subject><subject>Cell membranes</subject><subject>Deactivation</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - radiation effects</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Lipids</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Fluorescence</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Rhodamines - chemistry</subject><subject>Rhodamines - radiation effects</subject><subject>Signaling</subject><subject>Tethering</subject><subject>Ultraviolet Rays</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kc1LxDAQxYMo7vpx8a5UvIhQTZukTbxJ1VVYEETBW0nzsXRJm5q0gv-9qbuu4MG5zAzvx2N4A8BRAi8TiNiVhEJAnOBssQWmCcpwTDB92x5nwuIcYTIBe94vYaiE0F0wQSklOWTpFFw_qw_lfF0ZFfmO9zU3EW9l1Kumsy4swra9syayOjJ1V8vI14uWm7pdHIAdzY1Xh-u-D17v716Kh3j-NHssbuaxQBnqY0GUxJmSikGqOYUS5oroHPMcUiF0LiqGMIcaEoVQzgSpMo1SRDTCKUE0R_vgfOXbOfs-KN-XTe2FMoa3yg6-TDGiGcUJSwN69gdd2sGFc0cKpyyEBEfDixUlnPXeKV12rm64-ywTWI6JlrewKL4TnQX4ZG05VI2SG_QnwgAcrwDnxUb9fUnQT__Ty05q9AUnPYR8</recordid><startdate>20200915</startdate><enddate>20200915</enddate><creator>Gaur, Pankaj</creator><creator>Galkin, Maksym</creator><creator>Hauke, Sebastian</creator><creator>Redkin, Ruslan</creator><creator>Barnes, Carolyn</creator><creator>Shvadchak, Volodymyr V</creator><creator>Yushchenko, Dmytro A</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0355-6197</orcidid><orcidid>https://orcid.org/0000-0001-8302-8073</orcidid></search><sort><creationdate>20200915</creationdate><title>Reversible spatial and temporal control of lipid signaling</title><author>Gaur, Pankaj ; 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These molecules were prepared by tethering
ortho
-nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They permit two sequential photocontrolled reactions, which are uncaging of a lipid analogue and then its repeated activation and deactivation. We used these molecules to activate GPR40 receptor transiently expressed in HeLa cells and demonstrated downstream modulation of intracellular Ca
2+
levels.
Fluorescent cages for selective delivery of photoswitchable lipids to specific cell membranes for high-precision studies of lipid signaling.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>32857092</pmid><doi>10.1039/d0cc04146g</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-0355-6197</orcidid><orcidid>https://orcid.org/0000-0001-8302-8073</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2020-09, Vol.56 (73), p.1646-1649 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_pubmed_primary_32857092 |
| source | Royal Society of Chemistry |
| subjects | Azo compounds Azo Compounds - chemistry Azo Compounds - radiation effects Calcium - metabolism Calcium ions Cell membranes Deactivation Fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - radiation effects Green Fluorescent Proteins - metabolism HeLa Cells Humans Lipids Microscopy, Confocal Microscopy, Fluorescence Receptors, G-Protein-Coupled - metabolism Rhodamines - chemistry Rhodamines - radiation effects Signaling Tethering Ultraviolet Rays |
| title | Reversible spatial and temporal control of lipid signaling |
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