Isolation of bioactive components with soluble epoxide hydrolase inhibitory activity from Stachys sieboldii MiQ. by ultrasonic-assisted extraction optimized using response surface methodology

• Published in: Preparative biochemistry & biotechnology 2021, Vol.51 (4), p.395-404
• Main Authors: Gao, Dan, Le Ba, Vinh, Rustam, Rustamov, Cho, Chong Woon, Yang, Seo Young, Su, Xiang Dong, Kim, Young Ho, Kang, Jong Seong
• Format: Article
• Language: English
• Subjects: Acetic acid; Bioactive compounds; Biological activity; Cardiovascular diseases; Dementia disorders; Energy conservation; Epoxide hydrolase; Herbal medicine; High performance liquid chromatography; HPLC-ESI-MS/MS; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medicinal plants; Photodiodes; Prediction models; Response surface methodology; sEH activity; Stachys; Stachys sieboldii MiQ; Staphylococcal enterotoxin H; Tryptophan; Ultraviolet detectors
• ISSN: 1082-6068; 1532-2297
• DOI: 10.1080/10826068.2020.1821217

Stachys sieboldii MiQ (SSM) is an important food and medicinal herb in Korea, used to improve memory of patients with senile dementia and cardiovascular diseases. However, little information on bioactive components from SSM or standardized extraction methods for these components is available. This study isolated and purified major components from SSM for the first time, and assessed their ability to inhibit soluble epoxide hydrolase (sEH). The results showed that acteoside is the most potent inhibitor of sEH, with an IC 50 of 33.5 ± 0.5 μM. Additional active components, including harpagide, tryptophan, and 8-acetate-harpagide, along with acteoside, were tentatively identified using high-performance liquid chromatography photodiode array tandem mass spectrometry (HPLC-PDA-MS/MS) and quantified using an ultraviolet detector at 210 nm. Further, an ultrasonic-assisted extraction technique for extraction of four bioactive compounds in SSM was developed and optimized using response surface methodology (RSM). The optimal extraction conditions were: extraction time, 30.46 minutes; extraction temperature, 67.95 °C, and methanol concentration 53.85%. The prediction model of RSM was validated with laboratory experiments. The similarity between predicted and actual values was 97.84%. The extraction method is thus a rapid, environment-friendly, energy-saving method can be applied to extract bioactive components from SSM in large quantities.