Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesi...

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Bibliographic Details
Published in:Soft matter 2020-01, Vol.16 (39), p.8996-91
Main Authors: Faizi, Hammad A, Reeves, Cody J, Georgiev, Vasil N, Vlahovska, Petia M, Dimova, Rumiana
Format: Article
Language:English
Subjects:
Bending modulus
Confocal microscopy
Membranes
Microscopes
Microscopy
Phase contrast
Rigidity
Spectroscopy
Spectrum analysis
Vesicles
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Summary:A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies. Bending rigidity measured with fluctuation spectroscopy is insensitive to the imaging technique: do's and do not's when using confocal and phase contrast imaging.
ISSN:1744-683X
1744-6848
DOI:10.1039/d0sm00943a