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Lobelia trigona Roxb-based nanomedicine with enhanced biological applications: in vitro and in vivo approach
This is the first study to report the green synthesis of Lobelia trigona Roxb-mediated silver nanoparticles (LTAgNPs). The optical and structural properties of the synthesised LTAgNPs were analysed using ultraviolet–visible spectroscopy, scanning electron microscopy, Fourier transform infrared, dyna...
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| Published in: | IET nanobiotechnology 2020-10, Vol.14 (8), p.739-748 |
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| container_title | IET nanobiotechnology |
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| creator | Samdavid Thanapaul, Rex Jeya Rajkumar Manikandan, Sreeraj K Govindaraj, Tamil Selvan Selvakumar, Paulraj Mosae Enoch, Israel V.M.V Muthukumar Nadar, Meenakshi Subbaraman Adyanpuram |
| description | This is the first study to report the green synthesis of Lobelia trigona Roxb-mediated silver nanoparticles (LTAgNPs). The optical and structural properties of the synthesised LTAgNPs were analysed using ultraviolet–visible spectroscopy, scanning electron microscopy, Fourier transform infrared, dynamic light scattering and energy dispersive X-ray. LTAgNps were evaluated for their anti-bacterial and anti-fungal properties against 18 pathogens and exhibited significant inhibition against all the strains tested. LTAgNPs had potential scavenging effects on the DPPH, •OH, O2•− free radical scavenging assays and reducing power assay. LTAgNps possess strong anti-cancer activity against five human cancer cell lines (A549, MCF-7, MDA-MB-231, HeLa and KB) in a dose-dependent manner. The antiproliferative, anti-inflammatory and genotoxicity effects of LTAgNPs were further confirmed by the lactate dehydrogenase release assay, nitric oxide inhibitory assay and comet assay. Furthermore, the incision, excision and burn wound-healing activity of formulated LTAgNPs ointment was assessed in rats. All the wounds had significant healing in groups treated with LTAgNPs ointment compared to the groups treated with the commonly prescribed ointment (SilverexTM). This study shows and suggests that the previously unreported LTAgNPs could be used as a nanomedicine with significant biological applications. |
| doi_str_mv | 10.1049/iet-nbt.2020.0148 |
| format | article |
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The optical and structural properties of the synthesised LTAgNPs were analysed using ultraviolet–visible spectroscopy, scanning electron microscopy, Fourier transform infrared, dynamic light scattering and energy dispersive X-ray. LTAgNps were evaluated for their anti-bacterial and anti-fungal properties against 18 pathogens and exhibited significant inhibition against all the strains tested. LTAgNPs had potential scavenging effects on the DPPH, •OH, O2•− free radical scavenging assays and reducing power assay. LTAgNps possess strong anti-cancer activity against five human cancer cell lines (A549, MCF-7, MDA-MB-231, HeLa and KB) in a dose-dependent manner. The antiproliferative, anti-inflammatory and genotoxicity effects of LTAgNPs were further confirmed by the lactate dehydrogenase release assay, nitric oxide inhibitory assay and comet assay. Furthermore, the incision, excision and burn wound-healing activity of formulated LTAgNPs ointment was assessed in rats. All the wounds had significant healing in groups treated with LTAgNPs ointment compared to the groups treated with the commonly prescribed ointment (SilverexTM). This study shows and suggests that the previously unreported LTAgNPs could be used as a nanomedicine with significant biological applications.</description><identifier>ISSN: 1751-8741</identifier><identifier>ISSN: 1751-875X</identifier><identifier>EISSN: 1751-875X</identifier><identifier>DOI: 10.1049/iet-nbt.2020.0148</identifier><identifier>PMID: 33108332</identifier><language>eng</language><publisher>United States: The Institution of Engineering and Technology</publisher><subject>A549 human cancer cell lines ; Animals ; Anti-Bacterial Agents - administration & dosage ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; antibacterial activity ; antibacterial properties ; anticancer activity ; antifungal properties ; antiinflammatory effects ; Antineoplastic Agents - administration & dosage ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - pharmacology ; Antioxidants - administration & dosage ; Antioxidants - chemistry ; Antioxidants - pharmacology ; antiproliferative effects ; Bacteria - drug effects ; biochemistry ; biological applications ; biomedical materials ; burn wound‐healing activity ; Burns - drug therapy ; Burns - pathology ; cancer ; cellular biophysics ; comet assay ; DNA Damage ; dynamic light scattering ; energy dispersive X‐ray analysis ; enzymes ; excision ; formulated LTAgNPs ointment ; Fourier transform infrared spectra ; Fourier transform infrared spectroscopy ; free radical scavenging ; Fungi - drug effects ; genotoxicity effects ; HeLa human cancer cell lines ; in vitro approach ; In Vitro Techniques ; in vivo approach ; lactate dehydrogenase release assay ; light scattering ; Lobelia - chemistry ; Lobelia trigona Roxb‐based nanomedicine ; Lobelia trigona Roxb‐mediated silver nanoparticles ; Male ; MCF‐7 human cancer cell lines ; MDA‐MB‐231 human cancer cell lines ; Metal Nanoparticles - administration & dosage ; Metal Nanoparticles - chemistry ; microorganisms ; molecular biophysics ; nanofabrication ; Nanomedicine ; nanoparticles ; Neoplasms - drug therapy ; Neoplasms - pathology ; nitric oxide inhibitory assay ; optical properties ; pathogens ; Plant Extracts - pharmacology ; power assay ; Rats ; Rats, Wistar ; Research Article ; scanning electron microscopy ; scavenging effects ; silver ; Silver - chemistry ; strains ; structural properties ; toxicology ; Tumor Cells, Cultured ; ultraviolet spectra ; ultraviolet‐visible spectroscopy ; visible spectra ; Wound Healing - drug effects ; wounds</subject><ispartof>IET nanobiotechnology, 2020-10, Vol.14 (8), p.739-748</ispartof><rights>The Institution of Engineering and Technology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5220-73b6faf35c7a6f80e805541e3545cde3fccc6a007024bd1509250d8995b7acc23</citedby><cites>FETCH-LOGICAL-c5220-73b6faf35c7a6f80e805541e3545cde3fccc6a007024bd1509250d8995b7acc23</cites><orcidid>0000-0003-1584-7661</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8676277/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8676277/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,9755,11562,27924,27925,46052,46476,53791,53793</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1049%2Fiet-nbt.2020.0148$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33108332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Samdavid Thanapaul, Rex Jeya Rajkumar</creatorcontrib><creatorcontrib>Manikandan, Sreeraj K</creatorcontrib><creatorcontrib>Govindaraj, Tamil Selvan</creatorcontrib><creatorcontrib>Selvakumar, Paulraj Mosae</creatorcontrib><creatorcontrib>Enoch, Israel V.M.V</creatorcontrib><creatorcontrib>Muthukumar Nadar, Meenakshi Subbaraman Adyanpuram</creatorcontrib><title>Lobelia trigona Roxb-based nanomedicine with enhanced biological applications: in vitro and in vivo approach</title><title>IET nanobiotechnology</title><addtitle>IET Nanobiotechnol</addtitle><description>This is the first study to report the green synthesis of Lobelia trigona Roxb-mediated silver nanoparticles (LTAgNPs). The optical and structural properties of the synthesised LTAgNPs were analysed using ultraviolet–visible spectroscopy, scanning electron microscopy, Fourier transform infrared, dynamic light scattering and energy dispersive X-ray. LTAgNps were evaluated for their anti-bacterial and anti-fungal properties against 18 pathogens and exhibited significant inhibition against all the strains tested. LTAgNPs had potential scavenging effects on the DPPH, •OH, O2•− free radical scavenging assays and reducing power assay. LTAgNps possess strong anti-cancer activity against five human cancer cell lines (A549, MCF-7, MDA-MB-231, HeLa and KB) in a dose-dependent manner. The antiproliferative, anti-inflammatory and genotoxicity effects of LTAgNPs were further confirmed by the lactate dehydrogenase release assay, nitric oxide inhibitory assay and comet assay. Furthermore, the incision, excision and burn wound-healing activity of formulated LTAgNPs ointment was assessed in rats. All the wounds had significant healing in groups treated with LTAgNPs ointment compared to the groups treated with the commonly prescribed ointment (SilverexTM). This study shows and suggests that the previously unreported LTAgNPs could be used as a nanomedicine with significant biological applications.</description><subject>A549 human cancer cell lines</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibacterial activity</subject><subject>antibacterial properties</subject><subject>anticancer activity</subject><subject>antifungal properties</subject><subject>antiinflammatory effects</subject><subject>Antineoplastic Agents - administration & dosage</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Antioxidants - administration & dosage</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>antiproliferative effects</subject><subject>Bacteria - drug effects</subject><subject>biochemistry</subject><subject>biological applications</subject><subject>biomedical materials</subject><subject>burn wound‐healing activity</subject><subject>Burns - drug therapy</subject><subject>Burns - pathology</subject><subject>cancer</subject><subject>cellular biophysics</subject><subject>comet assay</subject><subject>DNA Damage</subject><subject>dynamic light scattering</subject><subject>energy dispersive X‐ray analysis</subject><subject>enzymes</subject><subject>excision</subject><subject>formulated LTAgNPs ointment</subject><subject>Fourier transform infrared spectra</subject><subject>Fourier transform infrared spectroscopy</subject><subject>free radical scavenging</subject><subject>Fungi - drug effects</subject><subject>genotoxicity effects</subject><subject>HeLa human cancer cell lines</subject><subject>in vitro approach</subject><subject>In Vitro Techniques</subject><subject>in vivo approach</subject><subject>lactate dehydrogenase release assay</subject><subject>light scattering</subject><subject>Lobelia - chemistry</subject><subject>Lobelia trigona Roxb‐based nanomedicine</subject><subject>Lobelia trigona Roxb‐mediated silver nanoparticles</subject><subject>Male</subject><subject>MCF‐7 human cancer cell lines</subject><subject>MDA‐MB‐231 human cancer cell lines</subject><subject>Metal Nanoparticles - administration & dosage</subject><subject>Metal Nanoparticles - chemistry</subject><subject>microorganisms</subject><subject>molecular biophysics</subject><subject>nanofabrication</subject><subject>Nanomedicine</subject><subject>nanoparticles</subject><subject>Neoplasms - drug therapy</subject><subject>Neoplasms - pathology</subject><subject>nitric oxide inhibitory assay</subject><subject>optical properties</subject><subject>pathogens</subject><subject>Plant Extracts - pharmacology</subject><subject>power assay</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Research Article</subject><subject>scanning electron microscopy</subject><subject>scavenging effects</subject><subject>silver</subject><subject>Silver - chemistry</subject><subject>strains</subject><subject>structural properties</subject><subject>toxicology</subject><subject>Tumor Cells, Cultured</subject><subject>ultraviolet spectra</subject><subject>ultraviolet‐visible spectroscopy</subject><subject>visible spectra</subject><subject>Wound Healing - drug effects</subject><subject>wounds</subject><issn>1751-8741</issn><issn>1751-875X</issn><issn>1751-875X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkUtr3DAUhUVpadK0P6CbomW78EQPy_JkUUhCXjCkUKbQnbiW5RkFjeRKnknz7yvjZEihJStdcb9zdHUPQh8pmVFSzo-tGQrfDDNGGJkRWtav0CGVgha1FD9f7-uSHqB3Kd0RIoTg9Vt0wDklNefsELlFaIyzgIdoV8ED_h5-N0UDybTYgw8b01ptvcH3dlhj49fgdW41Nriwshochr53uRhs8OkEW493dogBg2-nyy6MSAyg1-_Rmw5cMh8ezyP04_JieX5dLL5d3ZyfLgotGCOF5E3VQceFllB1NTF1HrykhotS6NbwTmtdASGSsLJpqSBzJkhbz-eikaA140fo6-Tbb5v8AW38EMGpPtoNxAcVwKq_O96u1SrsVF3JikmZDT4_GsTwa2vSoDY2aeMceBO2SbFSCCrzAmlG6YTqGFKKpts_Q4kaU1I5JZVTUmNKakwpaz49n2-veIolAycTcG-deXjZUd2eLdnZZV6JIFn8ZRKP2F3YRp93rW4uliP1TNO3XWaLf7D_n_wP8znAHQ</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Samdavid Thanapaul, Rex Jeya Rajkumar</creator><creator>Manikandan, Sreeraj K</creator><creator>Govindaraj, Tamil Selvan</creator><creator>Selvakumar, Paulraj Mosae</creator><creator>Enoch, Israel V.M.V</creator><creator>Muthukumar Nadar, Meenakshi Subbaraman Adyanpuram</creator><general>The Institution of Engineering and Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1584-7661</orcidid></search><sort><creationdate>202010</creationdate><title>Lobelia trigona Roxb-based nanomedicine with enhanced biological applications: in vitro and in vivo approach</title><author>Samdavid Thanapaul, Rex Jeya Rajkumar ; Manikandan, Sreeraj K ; Govindaraj, Tamil Selvan ; Selvakumar, Paulraj Mosae ; Enoch, Israel V.M.V ; Muthukumar Nadar, Meenakshi Subbaraman Adyanpuram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5220-73b6faf35c7a6f80e805541e3545cde3fccc6a007024bd1509250d8995b7acc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>A549 human cancer cell lines</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - administration & dosage</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibacterial activity</topic><topic>antibacterial properties</topic><topic>anticancer activity</topic><topic>antifungal properties</topic><topic>antiinflammatory effects</topic><topic>Antineoplastic Agents - administration & dosage</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Antioxidants - administration & dosage</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - pharmacology</topic><topic>antiproliferative effects</topic><topic>Bacteria - drug effects</topic><topic>biochemistry</topic><topic>biological applications</topic><topic>biomedical materials</topic><topic>burn wound‐healing activity</topic><topic>Burns - drug therapy</topic><topic>Burns - pathology</topic><topic>cancer</topic><topic>cellular biophysics</topic><topic>comet assay</topic><topic>DNA Damage</topic><topic>dynamic light scattering</topic><topic>energy dispersive X‐ray analysis</topic><topic>enzymes</topic><topic>excision</topic><topic>formulated LTAgNPs ointment</topic><topic>Fourier transform infrared spectra</topic><topic>Fourier transform infrared spectroscopy</topic><topic>free radical scavenging</topic><topic>Fungi - drug effects</topic><topic>genotoxicity effects</topic><topic>HeLa human cancer cell lines</topic><topic>in vitro approach</topic><topic>In Vitro Techniques</topic><topic>in vivo approach</topic><topic>lactate dehydrogenase release assay</topic><topic>light scattering</topic><topic>Lobelia - chemistry</topic><topic>Lobelia trigona Roxb‐based nanomedicine</topic><topic>Lobelia trigona Roxb‐mediated silver nanoparticles</topic><topic>Male</topic><topic>MCF‐7 human cancer cell lines</topic><topic>MDA‐MB‐231 human cancer cell lines</topic><topic>Metal Nanoparticles - administration & dosage</topic><topic>Metal Nanoparticles - chemistry</topic><topic>microorganisms</topic><topic>molecular biophysics</topic><topic>nanofabrication</topic><topic>Nanomedicine</topic><topic>nanoparticles</topic><topic>Neoplasms - drug therapy</topic><topic>Neoplasms - pathology</topic><topic>nitric oxide inhibitory assay</topic><topic>optical properties</topic><topic>pathogens</topic><topic>Plant Extracts - pharmacology</topic><topic>power assay</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Research Article</topic><topic>scanning electron microscopy</topic><topic>scavenging effects</topic><topic>silver</topic><topic>Silver - chemistry</topic><topic>strains</topic><topic>structural properties</topic><topic>toxicology</topic><topic>Tumor Cells, Cultured</topic><topic>ultraviolet spectra</topic><topic>ultraviolet‐visible spectroscopy</topic><topic>visible spectra</topic><topic>Wound Healing - drug effects</topic><topic>wounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samdavid Thanapaul, Rex Jeya Rajkumar</creatorcontrib><creatorcontrib>Manikandan, Sreeraj K</creatorcontrib><creatorcontrib>Govindaraj, Tamil Selvan</creatorcontrib><creatorcontrib>Selvakumar, Paulraj Mosae</creatorcontrib><creatorcontrib>Enoch, Israel V.M.V</creatorcontrib><creatorcontrib>Muthukumar Nadar, Meenakshi Subbaraman Adyanpuram</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>IET nanobiotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Samdavid Thanapaul, Rex Jeya Rajkumar</au><au>Manikandan, Sreeraj K</au><au>Govindaraj, Tamil Selvan</au><au>Selvakumar, Paulraj Mosae</au><au>Enoch, Israel V.M.V</au><au>Muthukumar Nadar, Meenakshi Subbaraman Adyanpuram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lobelia trigona Roxb-based nanomedicine with enhanced biological applications: in vitro and in vivo approach</atitle><jtitle>IET nanobiotechnology</jtitle><addtitle>IET Nanobiotechnol</addtitle><date>2020-10</date><risdate>2020</risdate><volume>14</volume><issue>8</issue><spage>739</spage><epage>748</epage><pages>739-748</pages><issn>1751-8741</issn><issn>1751-875X</issn><eissn>1751-875X</eissn><abstract>This is the first study to report the green synthesis of Lobelia trigona Roxb-mediated silver nanoparticles (LTAgNPs). The optical and structural properties of the synthesised LTAgNPs were analysed using ultraviolet–visible spectroscopy, scanning electron microscopy, Fourier transform infrared, dynamic light scattering and energy dispersive X-ray. LTAgNps were evaluated for their anti-bacterial and anti-fungal properties against 18 pathogens and exhibited significant inhibition against all the strains tested. LTAgNPs had potential scavenging effects on the DPPH, •OH, O2•− free radical scavenging assays and reducing power assay. LTAgNps possess strong anti-cancer activity against five human cancer cell lines (A549, MCF-7, MDA-MB-231, HeLa and KB) in a dose-dependent manner. The antiproliferative, anti-inflammatory and genotoxicity effects of LTAgNPs were further confirmed by the lactate dehydrogenase release assay, nitric oxide inhibitory assay and comet assay. Furthermore, the incision, excision and burn wound-healing activity of formulated LTAgNPs ointment was assessed in rats. All the wounds had significant healing in groups treated with LTAgNPs ointment compared to the groups treated with the commonly prescribed ointment (SilverexTM). This study shows and suggests that the previously unreported LTAgNPs could be used as a nanomedicine with significant biological applications.</abstract><cop>United States</cop><pub>The Institution of Engineering and Technology</pub><pmid>33108332</pmid><doi>10.1049/iet-nbt.2020.0148</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1584-7661</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | A549 human cancer cell lines Animals Anti-Bacterial Agents - administration & dosage Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology antibacterial activity antibacterial properties anticancer activity antifungal properties antiinflammatory effects Antineoplastic Agents - administration & dosage Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Antioxidants - administration & dosage Antioxidants - chemistry Antioxidants - pharmacology antiproliferative effects Bacteria - drug effects biochemistry biological applications biomedical materials burn wound‐healing activity Burns - drug therapy Burns - pathology cancer cellular biophysics comet assay DNA Damage dynamic light scattering energy dispersive X‐ray analysis enzymes excision formulated LTAgNPs ointment Fourier transform infrared spectra Fourier transform infrared spectroscopy free radical scavenging Fungi - drug effects genotoxicity effects HeLa human cancer cell lines in vitro approach In Vitro Techniques in vivo approach lactate dehydrogenase release assay light scattering Lobelia - chemistry Lobelia trigona Roxb‐based nanomedicine Lobelia trigona Roxb‐mediated silver nanoparticles Male MCF‐7 human cancer cell lines MDA‐MB‐231 human cancer cell lines Metal Nanoparticles - administration & dosage Metal Nanoparticles - chemistry microorganisms molecular biophysics nanofabrication Nanomedicine nanoparticles Neoplasms - drug therapy Neoplasms - pathology nitric oxide inhibitory assay optical properties pathogens Plant Extracts - pharmacology power assay Rats Rats, Wistar Research Article scanning electron microscopy scavenging effects silver Silver - chemistry strains structural properties toxicology Tumor Cells, Cultured ultraviolet spectra ultraviolet‐visible spectroscopy visible spectra Wound Healing - drug effects wounds |
| title | Lobelia trigona Roxb-based nanomedicine with enhanced biological applications: in vitro and in vivo approach |
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